CAJ | 학술논문

目的评价海马单核细胞趋化因子1(MCP-1)及其受体(CCR2)与老龄大鼠术后认知功能障碍的关系.方法健康雄性SD大鼠48只,20 ～ 22月龄,体重480～ 550 g,采用随机数字表法,将其分为2组(n=24):对照组(C组)和术后认知功能障碍组(POCD组).POCD组吸入2.0％异氟醚麻醉,并行脾切除术.分别于术前、术后1、3和7d时行Morris水迷宫实验测定认知功能,记录逃避潜伏期和游泳距离.分别于术后1、3和7d水迷宫实验结束后处死8只大鼠,取海马组织,采用Western blot法测定MCP-1和CCR2的表达水平.结果与C组比较,POCD组术后1、3和7d时逃避潜伏期和游泳距离延长,海马组织MCP-1和CCR2表达上调(P＜0.05).结论老龄大鼠术后认知功能障碍发生的机制可能与海马MCP-1和CCR2表达上调有关.
목적 평개해마단핵세포추화인자1(MCP-1)급기수체(CCR2)여노령대서술후인지공능장애적관계.방법 건강웅성SD대서48지,20 ～ 22월령,체중480～ 550 g,채용수궤수자표법,장기분위2조(n=24):대조조(C조)화술후인지공능장애조(POCD조).POCD조흡입2.0％이불미마취,병행비절제술.분별우술전、술후1、3화7d시행Morris수미궁실험측정인지공능,기록도피잠복기화유영거리.분별우술후1、3화7d수미궁실험결속후처사8지대서,취해마조직,채용Western blot법측정MCP-1화CCR2적표체수평.결과 여C조비교,POCD조술후1、3화7d시도피잠복기화유영거리연장,해마조직MCP-1화CCR2표체상조(P＜0.05).결론 노령대서술후인지공능장애발생적궤제가능여해마MCP-1화CCR2표체상조유관.
Objective To evaluate the relationship between hippocampal monocyte chemotactic protein-1 (MCP-1) and its receptor C-C chemokine receptor 2 (CCR2) and postoperative cognitive dysfunction (POCD) in aged rats.Methods Forty-eight male Sprague-Dawley rats,aged 20-22 months,weighing 480-550 g,were randomly divided into 2 groups (n =24 each) using a random number table:control group (group C) and POCD group.POCD group inhaled 2.0％ isoflurane and underwent splenectomy.Before surgery and at 1,3 and 7 days after surgery,Morris water maze test was performed to evaluate the spatial learning and memory ability.The escape latency and swimming distance were recorded.Eight rats were sacrificed after the end of Morris water maze test performed at 1,3 and 7 days after surgery.Then the brains were removed,and the hippocampi were isolated for detection of the expression of MCP-1 and CCR2 by Western blot.Results Compared with group C,the escape latency and swimming distance were significantly prolonged,and the expression of MCP-1 and CCR2 in hippocampi was up-regulated at 1,3 and 7 days after surgery in POCD group.Conclusion Up-regulation of hippocampal MCP-1 and CCR2 expression may be involved in the mechanism of POCD in aged rats.