CAJ | 학술논문

目的评价钙蛋白酶在七氟醚麻醉诱发老龄大鼠海马神经元凋亡中的作用.方法健康雌性SD大鼠54只,18月龄,体重450～ 550 g,采用随机数字表法,将其分为3组(n=18):对照组(C组)、七氟醚组(Sev组)和钙蛋白酶抑制剂MDL28170组(M组).C组吸入50％O2-50％N2混合气体3 h;Sev组吸入3％七氟醚3 h;M组尾静脉注射MDL28170 10 mg/kg,30 min后吸入3％七氟醚3h,同时尾静脉输注MDL28170 3.33 mg·kg-1·h-1.每组取9只大鼠,分别于麻醉前30 min和麻醉后1～5d时采用Morris水迷宫实验评价认知功能,记录逃避潜伏期和穿越原平台次数;分别于麻醉前30 min和麻醉后1、5d水迷宫测试结束后,每组处死3只大鼠,取海马组织,采用流式细胞术测定神经元凋亡率和胞浆钙离子浓度.结果与C组比较,Sev组和M组麻醉后1d时逃避潜伏期延长,穿越原平台次数减少,神经元凋亡率和胞浆钙离子浓度升高(P＜0.05);与Sev组比较,M组麻醉后1d时逃避潜伏期缩短,穿越原平台次数增多,神经元凋亡率降低(P＜0.05),胞浆钙离子浓度差异无统计学意义(P＞0.05).结论钙蛋白酶激活参与了七氟醚麻醉诱发老龄大鼠海马神经元凋亡.
목적 평개개단백매재칠불미마취유발노령대서해마신경원조망중적작용.방법 건강자성SD대서54지,18월령,체중450～ 550 g,채용수궤수자표법,장기분위3조(n=18):대조조(C조)、칠불미조(Sev조)화개단백매억제제MDL28170조(M조).C조흡입50％O2-50％N2혼합기체3 h;Sev조흡입3％칠불미3 h;M조미정맥주사MDL28170 10 mg/kg,30 min후흡입3％칠불미3h,동시미정맥수주MDL28170 3.33 mg·kg-1·h-1.매조취9지대서,분별우마취전30 min화마취후1～5d시채용Morris수미궁실험평개인지공능,기록도피잠복기화천월원평태차수;분별우마취전30 min화마취후1、5d수미궁측시결속후,매조처사3지대서,취해마조직,채용류식세포술측정신경원조망솔화포장개리자농도.결과 여C조비교,Sev조화M조마취후1d시도피잠복기연장,천월원평태차수감소,신경원조망솔화포장개리자농도승고(P＜0.05);여Sev조비교,M조마취후1d시도피잠복기축단,천월원평태차수증다,신경원조망솔강저(P＜0.05),포장개리자농도차이무통계학의의(P＞0.05).결론 개단백매격활삼여료칠불미마취유발노령대서해마신경원조망.
Objective To evaluate the role of calpain in sevoflurane anesthesia-induced apoptosis in hippocampal neurons of aged rats.Methods Fifty-four healthy female Sprague-Dawley rats,aged 18 months,weighing 450-550 g,were randomly divided into 3 groups (n=12 each) using a random number table:control group (group C),sevoflurane group (Sev group) and calpain inhibitor M DL28170 group (group M).In group C,the rats inhaled 50％ O2-50％N2 for 3 h.In Sev group,the rats inhaled 3％ sevoflurane for 3 h.In group M,MDL28170 10 mg/kg was injected via the tail vein,30 min later 3％ sevoflurane was inhaled for 3 h,and MDL28170 was simultaneously infused at 3.33 mg · kg 1 · h-1 via the tail vein.Nine rats in each group were selected,and cognitive function was assessed by using Morris water maze test at 30 min before anesthesia and 1-5 days after anesthesia.The escape latency and frequency of crossing the original platform were recorded.After the end of Morris water maze test performed at 30 min before anesthesia and 1-5 days after anesthesia,3 rats in each group were sacrificed,and hippocampal tissues were obtained for detection of cell apoptosis (by flow cytometry) and intracellular [Ca2+] i.Apoptotic rate was calculated.Results Compared with group C,the escape latency was significantly prolonged,and the frequency of crossing the original platform was decreased,and the apoptotic rate and intracellular [Ca2+]i were increased at 1 day after anesthesia in Sev and M groups.Compared with group Sev,the escape latency was significantly shortened,the frequency of crossing the original platform was increased,and the apoptotic rate was decreased at 1 day after anesthesia,and no significant change was found in intracellular [Ca2+]i in group M.Conclusion Calpain activation is involved in sevoflurane anesthesia-induced apoptosis in hippocampal neurons of aged rats.