CAJ | 학술논문

目的探讨右美托咪定对内毒素血症大鼠急性肾损伤的影响.方法健康雄性清洁级SD大鼠30只,4～6月龄,体重180 ～ 220 g,采用随机数字表法分为3组(n=10):对照组(C组)、脂多糖(LPS)组(L组)和右美托咪定组(D组).L组静脉注射LPS 5 mg/kg制备内毒素血症模型.D组静脉注射LPS 5 mg/kg后,静脉注射右美托咪定负荷量7μg/kg,15 min后以5μg· kg-1·h-1的速率静脉输注6 h,L组和C组给予等容量生理盐水.LPS给药结束后6h时取股静脉血样,检测血清Cr、BUN、TNF-α和IL-6的浓度.LPS给药结束后24 h时处死动物,取肾组织,观察病理学结果,采用Western blot法测定紧密连接蛋白ZO-1和occludin的表达水平.结果与C组比较,L组和D组血清Cr、BUN、TNF-α和IL-6的浓度升高,肾组织ZO-1和occludin的表达下调(P＜0.05);与L组比较,D组血清Cr、BUN、TNF-α和IL-6的浓度降低,肾组织ZO-1和occludin的表达上调(P＜0.05).D组肾组织病理学损伤较L组减轻.结论右美托咪定可减轻内毒素血症大鼠急性肾损伤.
목적 탐토우미탁미정대내독소혈증대서급성신손상적영향.방법 건강웅성청길급SD대서30지,4～6월령,체중180 ～ 220 g,채용수궤수자표법분위3조(n=10):대조조(C조)、지다당(LPS)조(L조)화우미탁미정조(D조).L조정맥주사LPS 5 mg/kg제비내독소혈증모형.D조정맥주사LPS 5 mg/kg후,정맥주사우미탁미정부하량7μg/kg,15 min후이5μg· kg-1·h-1적속솔정맥수주6 h,L조화C조급여등용량생리염수.LPS급약결속후6h시취고정맥혈양,검측혈청Cr、BUN、TNF-α화IL-6적농도.LPS급약결속후24 h시처사동물,취신조직,관찰병이학결과,채용Western blot법측정긴밀련접단백ZO-1화occludin적표체수평.결과 여C조비교,L조화D조혈청Cr、BUN、TNF-α화IL-6적농도승고,신조직ZO-1화occludin적표체하조(P＜0.05);여L조비교,D조혈청Cr、BUN、TNF-α화IL-6적농도강저,신조직ZO-1화occludin적표체상조(P＜0.05).D조신조직병이학손상교L조감경.결론 우미탁미정가감경내독소혈증대서급성신손상.
Objective To investigate the effect of dexmedetomidine on acute kidney injury in endotoxemic rats.Methods Thirty adult male Sprague-Dawley rats,aged 4-6 months,weighing 180-220 g,were randomly divided into 3 groups (n =10 each) using a random number table:control group (group C),lipopolysaccharide group (group L),and dexmedetomidine (group D).Lipopolysaccharide (LPS) 5 mg/kg was injected slowly into the femoral vein to establish the model of endotoxemic in rats anesthetized with chloral hydrate.In group D,after LPS injection,a loading dose of dexmedetomidine 7 μg/kg was injected intravenously,and 15 min later dexmedetomidine was infused for 6 h at 5 μg · kg-1 · h-1,while the equal volume of normal saline was given in L and C groups.At 6 h after the end of LPS administration,blood samples were collected from the femoral vein for determination of serum creatinine (Cr),blood urea nitrogen (BUN),tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations.At 24 h after the end of LPS administration,the animals were sacrificed and kidneys were removed for microscopic examination and for determination of the expression of tight junction proteins ZO-1 and occludin in renal tissues by Western blot.Results Compared with group C,the serum Cr,BUN,TNF-α and IL-6 concentrations were significantly increased,and the expression of ZO-1 and occluding was down-regulated in L and D groups.Compared with group L,the serum Cr,BUN,TNF-α and IL-6 concentrations were significantly decreased,the expression of ZO-1 and occluding was up-regulated,and the pathological changes of kidneys were mitigated in D group.Conclusion Dexmedetomidine can alleviate acute kidney injury in endotoxemic rats.