食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
14期
4-7
,共4页
木犀草素%膜片钳技术%钙电流%心肌细胞
木犀草素%膜片鉗技術%鈣電流%心肌細胞
목서초소%막편겸기술%개전류%심기세포
luteolin%whole-cell patch clamp%calcium current%myocardial cells
研究木犀草素对大鼠心室肌细胞L型钙电流的影响.分离单个大鼠心室肌细胞,应用全细胞膜片钳技术测定钙电流.木犀草素能够剂量依赖性地降低缺氧心肌细胞L型钙电流:木犀草素小剂量组、中剂量组和大剂量组分别使Ica.L的峰值电流由给药前的(921±51) pA减小到给药后的(583.3±54) pA (P<0.05)、(491.2±47 )pA (P<0.05)和(276.3±43 )pA(P<0.05),降低Ca2+电流幅值的百分率分别为36.7%、46.7%和70%,而在药物被冲洗5 min后,L型钙电流几乎可恢复到正常水平.木犀草素能够剂量依赖性降低缺氧心室肌细胞的L型钙电流.
研究木犀草素對大鼠心室肌細胞L型鈣電流的影響.分離單箇大鼠心室肌細胞,應用全細胞膜片鉗技術測定鈣電流.木犀草素能夠劑量依賴性地降低缺氧心肌細胞L型鈣電流:木犀草素小劑量組、中劑量組和大劑量組分彆使Ica.L的峰值電流由給藥前的(921±51) pA減小到給藥後的(583.3±54) pA (P<0.05)、(491.2±47 )pA (P<0.05)和(276.3±43 )pA(P<0.05),降低Ca2+電流幅值的百分率分彆為36.7%、46.7%和70%,而在藥物被遲洗5 min後,L型鈣電流幾乎可恢複到正常水平.木犀草素能夠劑量依賴性降低缺氧心室肌細胞的L型鈣電流.
연구목서초소대대서심실기세포L형개전류적영향.분리단개대서심실기세포,응용전세포막편겸기술측정개전류.목서초소능구제량의뢰성지강저결양심기세포L형개전류:목서초소소제량조、중제량조화대제량조분별사Ica.L적봉치전류유급약전적(921±51) pA감소도급약후적(583.3±54) pA (P<0.05)、(491.2±47 )pA (P<0.05)화(276.3±43 )pA(P<0.05),강저Ca2+전류폭치적백분솔분별위36.7%、46.7%화70%,이재약물피충세5 min후,L형개전류궤호가회복도정상수평.목서초소능구제량의뢰성강저결양심실기세포적L형개전류.
To explore the effect of luteolin on L-type calcium current in rat ventricular muscle cells. The single rat ventricular cell was seperated,and the technique of whole-cell patch clamp was apply to detect the calcium current. The results showed that luteolin dose dependently inhibited the L-type calcium current in hypoxia treated ventricular myocytes of the rats:in low dosage group,middle dosage group and high dosage group, the peak of Ica.L respectively reduced from (921±51) pA before treatment to (583.3±54) pA(P<0.05),(491.2±47) pA(P<0.05) and (276.3±43) pA (P<0.05) after treatment, the value of electric current Ca2+that decrease in percentage was 36.7%,46.7%and 70%, respectively, and the electric current Ca2+could almost return to the normal level after luteolin was washout. Luteolin could dose-related inhibit the L-type calcium current in rat hypoxia treated ventricular myocytes.
