CAJ | 학술논문

目的：建立豚鼠耳蜗宏观生物数值模型。方法取成年豚鼠1只，进行电耳镜、声导抗、听性脑干反应、耳声发射及内耳薄层CT扫描，除外耳部疾患及内耳畸形，全麻断头后取耳蜗，经石蜡包埋、连续切片（6μm）、HE染色、摄像等步骤获得一整套豚鼠内耳组织切片的图像资料；利用Photoshop软件进行去色、调整灰度及对比度等处理，利用mimics软件识别边界并标定，获得边界点的三维坐标，将坐标导入Hypermesh软件建立网格化模型，利用ANASYS软件建立耳蜗膜迷路的三维生物数值模型，基于该模型进行基底膜的模态分析。结果所建豚鼠耳蜗生物数值模型能够清晰显示耳蜗的空间结构特性；生物数值模拟显示了耳蜗基底膜前10阶感音频率特异性，与“行波学说”结果一致。结论运用组织连续切片技术并结合有限元分析软件建立耳蜗宏观生物数值模型的方法可行，所建模型可用于耳蜗感音功能研究。
목적：건립돈서이와굉관생물수치모형。방법취성년돈서1지，진행전이경、성도항、은성뇌간반응、이성발사급내이박층CT소묘，제외이부질환급내이기형，전마단두후취이와，경석사포매、련속절편（6μm）、HE염색、섭상등보취획득일정투돈서내이조직절편적도상자료；이용Photoshop연건진행거색、조정회도급대비도등처리，이용mimics연건식별변계병표정，획득변계점적삼유좌표，장좌표도입Hypermesh연건건립망격화모형，이용ANASYS연건건립이와막미로적삼유생물수치모형，기우해모형진행기저막적모태분석。결과소건돈서이와생물수치모형능구청석현시이와적공간결구특성；생물수치모의현시료이와기저막전10계감음빈솔특이성，여“행파학설”결과일치。결론운용조직련속절편기술병결합유한원분석연건건립이와굉관생물수치모형적방법가행，소건모형가용우이와감음공능연구。
Objective To establish a macro-biomechanical model of the guinea pig's cochlea.Methods Both cochlea, abstracted from bilateral temporal bones of a healthy adult guinea pig, which was tested firstly including a series of examina-tion including electric otoscopy, acoustic immittance,brainstem auditory evoked potential, otoacoustic emissions, CT scan and so on.And then, a whole set of inner ear tissue slices images was obtained after a series of operations including embed-ding, slicing ( thickness 6μm) , immobilization, coloretur, photographing and so on.The images were processed by Photo-shop software, mimics software and Hypermesh software sequencely and a biomechanical model of the cochlea was obtained with ANSYS13.0 eventually.Based on the model, the modal analysis was done to test the accuracy of the model.Results Spatial structural feature of the reconstructed biomechanical model of the guinea pig's cochlea was displayed typically and the result of the model analysis proceeded on the macro-biomechanical model was the same as traveling wave theory.Con-clusion It is feasible technically to reconstruct biomechanical model of the cochlea with the tissue slices combining with the finite element soft wares.And the macro-biomechanical model could be applied to study the sensing process of cochlea.