CAJ | 학술논문

以老芒麦种子为材料，利用酶解－火焰干燥法来分析有丝分裂中期染色体核型，为老芒麦系统分类及育种工作提供科学依据。研究结果表明，老芒麦染色体数目为28条，13号染色体上有1对随体，臂比值大于2的染色体占7．14％，最长染色体与最短染色体比值为2．04，核型公式是2n ＝4x ＝28＝24m＋4sm（2SAT），不对称类型为2B。细胞有丝分裂呈现出间期、前期、前中期、中期、后期5个不同的时期。在分裂间期，细胞核染色均匀；到分裂前期时可以看到纤细状的网状染色体；进入分裂前中期，可辨别单个染色体；到分裂中期，染色体高度浓缩，姊妹染色单体及着丝点都清晰可辨；在分裂后期，姊妹染色单体分离。
이로망맥충자위재료，이용매해－화염간조법래분석유사분렬중기염색체핵형，위로망맥계통분류급육충공작제공과학의거。연구결과표명，로망맥염색체수목위28조，13호염색체상유1대수체，비비치대우2적염색체점7．14％，최장염색체여최단염색체비치위2．04，핵형공식시2n ＝4x ＝28＝24m＋4sm（2SAT），불대칭류형위2B。세포유사분렬정현출간기、전기、전중기、중기、후기5개불동적시기。재분렬간기，세포핵염색균균；도분렬전기시가이간도섬세상적망상염색체；진입분렬전중기，가변별단개염색체；도분렬중기，염색체고도농축，자매염색단체급착사점도청석가변；재분렬후기，자매염색단체분리。
A study has been undertaken to determine the karyotype of Elymus sibiricus .The results showed that the chromosome number of E .sibiricus was 28,with a pair of satellites on the short arms of the 13 th chro-mosomes.Its karyotype formula is 2n =4x =24m+4sm (2SAT).The percentage of chromosomes with arm ratios over 2 was 7.14%,and the ratio of the longest to the shortest was 2.04.Its karyotype asymmetry is 2B. There were five phases (Interphase,Prophase,Prometaphase,Metaphase and Anaphase)in the process of mi-tosis.At interphase the nuclei were dyed homogeneously.During prophase the shape of the slender chromo-somes was visible.The individual chromosome could be identified clearly in the prometaphase.At metaphase the chromosomes highly concentrated and the chromatids and centromeres were distinctly visible.During ana-phase the chromatids separated and moved to the opposite poles.