CAJ | 학술논문

2013年8月下旬，对甘肃天祝高寒草甸草原孢囊线虫发生情况进行调查，从嵩草和矮嵩草根部和根际土样中分离得到孢囊线虫。通过对孢囊和孢囊阴门锥的形态观察，初步鉴定为禾谷孢囊线虫（Heterodera avenae ）。其特征为孢囊浅褐色，阔柠檬形，阴门锥双膜孔，未见阴门下桥，泡状突明显。利用通用引物 AB28和 TW81对其群体rDNA-ITS 区进行 PCR 扩增和序列测定，得到 ITS 区序列长度为1045 bp。将所测得序列在 Genbank 和 Blast 上比对，结果显示，此禾谷孢囊线虫群体与 H ．avenae 群体相似度达99％～100％。进一步证实寄生于甘肃天祝高寒草甸草原嵩草和矮嵩草的孢囊线虫均为 H ．avenae ，这是首次报道莎草科嵩草和矮嵩草也是禾谷孢囊线虫的寄主。
2013년8월하순，대감숙천축고한초전초원포낭선충발생정황진행조사，종숭초화왜숭초근부화근제토양중분리득도포낭선충。통과대포낭화포낭음문추적형태관찰，초보감정위화곡포낭선충（Heterodera avenae ）。기특정위포낭천갈색，활저몽형，음문추쌍막공，미견음문하교，포상돌명현。이용통용인물 AB28화 TW81대기군체rDNA-ITS 구진행 PCR 확증화서렬측정，득도 ITS 구서렬장도위1045 bp。장소측득서렬재 Genbank 화 Blast 상비대，결과현시，차화곡포낭선충군체여 H ．avenae 군체상사도체99％～100％。진일보증실기생우감숙천축고한초전초원숭초화왜숭초적포낭선충균위 H ．avenae ，저시수차보도사초과숭초화왜숭초야시화곡포낭선충적기주。
A survey of grass cyst nematodes in Tianzhu alpine meadow steppe,Gansu Province was conducted in August,2013.Cyst nematodes were isolated from the roots and root rhizosphere of Kobresia sp.and Kobresia humilis .The nematodes were initially identified as Heterodera avenae based on cyst characteristics,particular-ly the cyst vulva cones.The cysts were light brown,large lenmon-shaped and contained vulva cones with bifenestrate and obvious bullae and without a vulva under-bridge.The 1045 bp sequence fragment of ITS region was amplified using PCR with general primers AB28 and TW81.The similarities between these groups and H . avena were 99%-100%.Molecular analysis confirmed that the cyst nematodes isolated from Kobresia sp.and Kobresia humilis growing in Tianzhu alpine meadow steppe were H .avenae .This is the first report identifying Kobresia sp.and Kobresia humilis as hosts of H .avenae .