CAJ | 학술논문

基因 mRNA 水平相对表达量的显著变化是二斑叶螨对拟除虫菊酯类药剂产生抗性的重要机制。为了揭示二斑叶螨对甲氰菊酯抗性产生的解毒酶分子机理，本研究采用实时荧光定量 PCR（quantitative real time PCR，qRT－PCR）方法分析二斑叶螨实验室敏感（SS）和田间种群（LZ-R、GN-R、WW-R、TS-R 和 LX-R）主要解毒酶谷胱甘肽转移酶（glutathione s-transferases，GSTs）、细胞色素 P450单加氧酶（cytocheome P450 monooxygenases，P450s）及羧酸酯酶（carboxyl／cholinesterases，CCEs）基因 mRNA 水平相对表达量的差异。结果表明，二斑叶螨不同种群不同解毒酶基因的相对表达量不同。WW-R 和 TS-R 种群中 TuGSTd05以及 LX-R 种群中 TuGSTd01和 TuG-STd06基因表达量均显著上调，为 SS 种群的1．42～2．34倍，而 GN-R 种群中 TuGSTd04，LZ-R 种群中 TuGSTd05和 GSTd09表达量显著下调，为 SS 种群的0．41～0．70倍；P450s 基因 CYP406A1和 CYP4CL1表达量在 LZ-R、GN-R 以及 WW-R 种群中均显著上调，分别为 SS 种群的1．80～4．88倍，此外，CYP 387A1在 LZ-R 种群中显著上调2．19倍，而在 LX-R 种群中显著下调0．42倍；CCEs 基因 TuCCE-35表达量在 WW-R 和 TS-R 种群中显著上调，分别为 SS 种群的2．82和3．09倍，而 TuCCE-36基因在所有种群中的表达量均不显著。二斑叶螨不同种群中解毒酶基因 GSTs、P450s 和 CCEs 的显著上调或下调可能与甲氰菊酯的抗性形成有关。
기인 mRNA 수평상대표체량적현저변화시이반협만대의제충국지류약제산생항성적중요궤제。위료게시이반협만대갑청국지항성산생적해독매분자궤리，본연구채용실시형광정량 PCR（quantitative real time PCR，qRT－PCR）방법분석이반협만실험실민감（SS）화전간충군（LZ-R、GN-R、WW-R、TS-R 화 LX-R）주요해독매곡광감태전이매（glutathione s-transferases，GSTs）、세포색소 P450단가양매（cytocheome P450 monooxygenases，P450s）급최산지매（carboxyl／cholinesterases，CCEs）기인 mRNA 수평상대표체량적차이。결과표명，이반협만불동충군불동해독매기인적상대표체량불동。WW-R 화 TS-R 충군중 TuGSTd05이급 LX-R 충군중 TuGSTd01화 TuG-STd06기인표체량균현저상조，위 SS 충군적1．42～2．34배，이 GN-R 충군중 TuGSTd04，LZ-R 충군중 TuGSTd05화 GSTd09표체량현저하조，위 SS 충군적0．41～0．70배；P450s 기인 CYP406A1화 CYP4CL1표체량재 LZ-R、GN-R 이급 WW-R 충군중균현저상조，분별위 SS 충군적1．80～4．88배，차외，CYP 387A1재 LZ-R 충군중현저상조2．19배，이재 LX-R 충군중현저하조0．42배；CCEs 기인 TuCCE-35표체량재 WW-R 화 TS-R 충군중현저상조，분별위 SS 충군적2．82화3．09배，이 TuCCE-36기인재소유충군중적표체량균불현저。이반협만불동충군중해독매기인 GSTs、P450s 화 CCEs 적현저상조혹하조가능여갑청국지적항성형성유관。
The variation of mRNA gene expression is an important pyrethroid insecticide resistance mechanism in Tetranychus urticae .To identify the molecular mechanism for producing detoxification enzymes in T.urti-caein response to fenpropathrin,the mRNA expression levels of glutathione s-transferases (GSTs),cyto-chrome (P450s)and carboxyl/cholinesterases (CCEs)genes in laboratory fenpropathrin susceptible (SS),fen-propathrin resistant (Sp-R)and field strains (LZ-R,GN-R,WW-R,TS-R and LX-R)of T.urticae were measured using quantitative real time PCR (qRT-PCR).The results showed that relative expression levels of <br> different detoxification enzyme genes varied with different strains of T.urticae .Compared with the SS strain, the expression levels of TuGSTd05 in the WW-R and TS-R strains,TuGSTd01 and TuGSTd06 in the LX-R strain were significantly up-regulated,1.42 -2.34 times greater than the SS strain,while TuGSTd04 in the GN-Rstrain,TuGSTd05 and TuGSTd09 in the LX-R strain were significantly down-regulated,0.41 -0.70 that of the SS strain.The expression levels of P450s genes CYP406A1 and CYP4CL1 in the LZ-R,GN-R and WW-R strains were significantly up-regulated,1.80-4.88 times that of the SS strain.The expression level of P450s genes CYP 387A1 in the LZ-R strain was also significantly higher (2.19 times)than the SS strain where-as that in the LX-R strain was significantly lower (0.42 times)than the SS strain.Similarly,the expression levels of CCEs gene TuCCE-35 in the WW-R and TS-R strains was significantly higher than the SS strain. However,the gene expression levels of TuCCE-36 did not change in all strains.The significant up-regulation or down-regulation of detoxification enzyme genes (GSTs,p450s and CCEs)among different strains of T.ur-ticae may be associated with the formation of resistance to fenpropathrin.