医学检验与临床
醫學檢驗與臨床
의학검험여림상
MEDICAL LATORATORY SCIENCE AND CLINICES
2015年
3期
3-5
,共3页
伍启康%吴奎海%李炜煊%崔东岚
伍啟康%吳奎海%李煒煊%崔東嵐
오계강%오규해%리위훤%최동람
肺炎克雷伯菌%ESBLs%整合子%ISCR1%ERIC-PCR
肺炎剋雷伯菌%ESBLs%整閤子%ISCR1%ERIC-PCR
폐염극뢰백균%ESBLs%정합자%ISCR1%ERIC-PCR
Klebsiella pneumoniae%ESBLs. Integrating the son%ISCR1%ERIC - PCR
目的：对临床分离产ESBLs肺炎克雷伯菌相关基因进行研究，并对其进行基因分型。方法：临床分离295株肺炎克雷伯菌，ESBLs药敏试验采用K-B法。PCR法检测I类整合酶、I类整合子基因盒、ISCR1、blaTEM、blaSHV、blaCTX-M。I类整合子PCR扩增阳性产物送去测序，测序结果在GenBank中用blastn进行核酸序列同源性搜索。菌株基因分型采用ERIC-PCR法。结果：产ESBLs肺炎克雷伯菌阳性率为21.3%。在63株产ESBLs肺炎克雷伯菌中，分为35个基因型，I类整合酶、ISCR1、blaTEM、blaSHV、blaCTX-M的阳性率分别为46.0%、12.7%、55.6%、0、81.0%，27个I类整合子含有7种类型基因盒。结论：在产ESBLs肺炎克雷伯菌中，I类整合子携带率较高，ESBLs基因型以blaTEM和blaCTX-M为主。我院产ESBLs肺炎克雷伯菌存在某种流行株。
目的：對臨床分離產ESBLs肺炎剋雷伯菌相關基因進行研究，併對其進行基因分型。方法：臨床分離295株肺炎剋雷伯菌，ESBLs藥敏試驗採用K-B法。PCR法檢測I類整閤酶、I類整閤子基因盒、ISCR1、blaTEM、blaSHV、blaCTX-M。I類整閤子PCR擴增暘性產物送去測序，測序結果在GenBank中用blastn進行覈痠序列同源性搜索。菌株基因分型採用ERIC-PCR法。結果：產ESBLs肺炎剋雷伯菌暘性率為21.3%。在63株產ESBLs肺炎剋雷伯菌中，分為35箇基因型，I類整閤酶、ISCR1、blaTEM、blaSHV、blaCTX-M的暘性率分彆為46.0%、12.7%、55.6%、0、81.0%，27箇I類整閤子含有7種類型基因盒。結論：在產ESBLs肺炎剋雷伯菌中，I類整閤子攜帶率較高，ESBLs基因型以blaTEM和blaCTX-M為主。我院產ESBLs肺炎剋雷伯菌存在某種流行株。
목적：대림상분리산ESBLs폐염극뢰백균상관기인진행연구，병대기진행기인분형。방법：림상분리295주폐염극뢰백균，ESBLs약민시험채용K-B법。PCR법검측I류정합매、I류정합자기인합、ISCR1、blaTEM、blaSHV、blaCTX-M。I류정합자PCR확증양성산물송거측서，측서결과재GenBank중용blastn진행핵산서렬동원성수색。균주기인분형채용ERIC-PCR법。결과：산ESBLs폐염극뢰백균양성솔위21.3%。재63주산ESBLs폐염극뢰백균중，분위35개기인형，I류정합매、ISCR1、blaTEM、blaSHV、blaCTX-M적양성솔분별위46.0%、12.7%、55.6%、0、81.0%，27개I류정합자함유7충류형기인합。결론：재산ESBLs폐염극뢰백균중，I류정합자휴대솔교고，ESBLs기인형이blaTEM화blaCTX-M위주。아원산ESBLs폐염극뢰백균존재모충류행주。
Objective:Of clinical separation ESBLs pneumonia klebsiella bacteria related genes, and genes.Methods:Clinical isolated 295 strains of pneumonia klebsiella bacteria, ESBLs drug sensitive test using the K - B method. PCR method to detect the I class integrase, class I integron gene box, ISCR1, blaTEM, blaSHV, blaCTX -m. Class I integron PCR amplification positive product sent to sequencing, the sequencing results in using blastn GenBank nucleic acid sequence homology search. Strain genotyping by ERIC - PCR method.Results: Producing ESBLs pneumonia klebsiella bacteria positive rate was 21.3%. In 63 strains producing ESBLs pneumonia klebsiella bacteria, divided into 35 genotypes, I class integrase, ISCR1, blaTEM, blaSHV, blaCTX - M positive rate were 46.0%, 12.7%, 55.6%, 0, 81.0%, 27 class I integron box containing the 7 types of gene.Conclusions:In producing ESBLs pneumonia klebsiella bacteria, class I integron carried rate is higher, ESBLs genotypes is given priority to with blaTEM and blaCTX - M. Our producing ESBLs pneumonia klebsiella bacteria exist some kind of epidemic strains.
