CAJ | 학술논문

寡核苷酸药物是近年来药物研发领域中的热点之一，相应分析方法的研究也被广泛开展，其中反相离子对色谱法以分离度高、易与MS对接等优势成为寡核苷酸分析中最常用的方法。本文综述了反相离子对色谱法在寡核苷酸分析方面的新进展，以下将从色谱柱、流动相，分离规律的探索，MS检测器方面进行系统介绍。
과핵감산약물시근년래약물연발영역중적열점지일，상응분석방법적연구야피엄범개전，기중반상리자대색보법이분리도고、역여MS대접등우세성위과핵감산분석중최상용적방법。본문종술료반상리자대색보법재과핵감산분석방면적신진전，이하장종색보주、류동상，분리규률적탐색，MS검측기방면진행계통개소。
Therapeutic oligonucleotides have been one of the hottest areas in R&D of drugs in recent years.The research of analysis method has been widely carried out.Ion-pairing reverse liquid chromatography is the most commonly used method for good resolution and easy docking with MS.A review is presented here on the progress of oligonucleotides analysis with ion-pairing reverse liquid chromatography.The column, mobile phase, MS detection, mechanism of analysis will be discussed.