草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2015年
8期
73-84
,共12页
赤霉素%乙烯利%花期%光合特性%抗氧化酶
赤黴素%乙烯利%花期%光閤特性%抗氧化酶
적매소%을희리%화기%광합특성%항양화매
gibberellin (GA3)%ethrel (CEPA)%flowering phase%photosynthetic characteristics%antioxidant enzymes
以香水百合为材料,采用盆栽试验,研究了在不同浓度赤霉素(GA3)、乙烯利(CEPA)的处理影响下,香水百合的花期(现蕾期、初花期、盛花期和末花期)变化、光合生理和抗氧化酶活性的变化及其花期响应。结果表明,1)在香水百合开花期,GA3和 CEPA 处理均不同程度地提高了香水百合株高、茎粗、蕾长和花径,并提前了初花期,延长了开花时间。GA3与株高、茎粗和蕾长呈显著正相关,对开花前中期影响显著,而 CEPA 与茎粗、蕾长和花径呈显著正相关,与末花天数呈显著负相关;2)GA3和 CEPA 处理提高了各时期光合色素含量,并有效缓解了末花期叶绿素的下降趋势,延长了光合作用时间;3)GA3和 CEPA 处理显著提高了香水百合开花期Pn 、Gs 、Tr 和Ls ,而 C i和 LUE 与其他参数呈负相关关系,分析表明,GA3和 CEPA 处理增强了花期叶片细胞的活性,提高了叶片的光合作用强度;4)GA3和 CEPA 处理的百合叶片 MDA 含量在开花各时期显著低于对照,抗氧化酶 SOD、CAT活性上升,同时,处理减缓了开花中后期 SOD 下降趋势,有效缓解了衰老进程,达到延长香水百合花期的目的。综合分析表明,GA3和 CEPA 提高了香水百合开花各时期的光合特性,并增强了抗氧化酶活性,进而对香水百合开花品质和开花花期有显著影响。其中,GA3200 mg/L+CEPA 30 mg/L 协同处理对提前花期和延长开花持续天数的调节效果最佳,其次为单水平 GA3200 mg/L 处理和单水平 CEPA 60 mg/L 处理,高浓度 GA3200 mg/L+CEPA 60 mg/L和 GA3300 mg/L+CEPA 30 mg/L 的调节作用下降。
以香水百閤為材料,採用盆栽試驗,研究瞭在不同濃度赤黴素(GA3)、乙烯利(CEPA)的處理影響下,香水百閤的花期(現蕾期、初花期、盛花期和末花期)變化、光閤生理和抗氧化酶活性的變化及其花期響應。結果錶明,1)在香水百閤開花期,GA3和 CEPA 處理均不同程度地提高瞭香水百閤株高、莖粗、蕾長和花徑,併提前瞭初花期,延長瞭開花時間。GA3與株高、莖粗和蕾長呈顯著正相關,對開花前中期影響顯著,而 CEPA 與莖粗、蕾長和花徑呈顯著正相關,與末花天數呈顯著負相關;2)GA3和 CEPA 處理提高瞭各時期光閤色素含量,併有效緩解瞭末花期葉綠素的下降趨勢,延長瞭光閤作用時間;3)GA3和 CEPA 處理顯著提高瞭香水百閤開花期Pn 、Gs 、Tr 和Ls ,而 C i和 LUE 與其他參數呈負相關關繫,分析錶明,GA3和 CEPA 處理增彊瞭花期葉片細胞的活性,提高瞭葉片的光閤作用彊度;4)GA3和 CEPA 處理的百閤葉片 MDA 含量在開花各時期顯著低于對照,抗氧化酶 SOD、CAT活性上升,同時,處理減緩瞭開花中後期 SOD 下降趨勢,有效緩解瞭衰老進程,達到延長香水百閤花期的目的。綜閤分析錶明,GA3和 CEPA 提高瞭香水百閤開花各時期的光閤特性,併增彊瞭抗氧化酶活性,進而對香水百閤開花品質和開花花期有顯著影響。其中,GA3200 mg/L+CEPA 30 mg/L 協同處理對提前花期和延長開花持續天數的調節效果最佳,其次為單水平 GA3200 mg/L 處理和單水平 CEPA 60 mg/L 處理,高濃度 GA3200 mg/L+CEPA 60 mg/L和 GA3300 mg/L+CEPA 30 mg/L 的調節作用下降。
이향수백합위재료,채용분재시험,연구료재불동농도적매소(GA3)、을희리(CEPA)적처리영향하,향수백합적화기(현뢰기、초화기、성화기화말화기)변화、광합생리화항양화매활성적변화급기화기향응。결과표명,1)재향수백합개화기,GA3화 CEPA 처리균불동정도지제고료향수백합주고、경조、뢰장화화경,병제전료초화기,연장료개화시간。GA3여주고、경조화뢰장정현저정상관,대개화전중기영향현저,이 CEPA 여경조、뢰장화화경정현저정상관,여말화천수정현저부상관;2)GA3화 CEPA 처리제고료각시기광합색소함량,병유효완해료말화기협록소적하강추세,연장료광합작용시간;3)GA3화 CEPA 처리현저제고료향수백합개화기Pn 、Gs 、Tr 화Ls ,이 C i화 LUE 여기타삼수정부상관관계,분석표명,GA3화 CEPA 처리증강료화기협편세포적활성,제고료협편적광합작용강도;4)GA3화 CEPA 처리적백합협편 MDA 함량재개화각시기현저저우대조,항양화매 SOD、CAT활성상승,동시,처리감완료개화중후기 SOD 하강추세,유효완해료쇠로진정,체도연장향수백합화기적목적。종합분석표명,GA3화 CEPA 제고료향수백합개화각시기적광합특성,병증강료항양화매활성,진이대향수백합개화품질화개화화기유현저영향。기중,GA3200 mg/L+CEPA 30 mg/L 협동처리대제전화기화연장개화지속천수적조절효과최가,기차위단수평 GA3200 mg/L 처리화단수평 CEPA 60 mg/L 처리,고농도 GA3200 mg/L+CEPA 60 mg/L화 GA3300 mg/L+CEPA 30 mg/L 적조절작용하강。
The process changes of flowering phase (the squaring stage,the early flowering stage,blooming stage,ending flowering)and the changes of photosynthetic characteristics,antioxidant enzymes,the flowering response of Lilium casa blanca were studied using the bulbs of L.casa blanca as pot experimental material un-der the coordination treatment of gibberellin (GA3 )and ethrel (CEPA).The results showed that,1 )It in-creased the plant height,thick stems,bud length and flower diameter,advanced the blooming stage and ex-tended the days of blossom of L.casa blanca under the treatment of GA3 and CEPA during the flowering <br> phase.There were significantly positive correlation between GA3 and plant height,stem diameter and bud length,while there were a significantly positive correlation between CEPA and stem diameter,bud length and flower diameter.GA3 made a significant influence in earlier and middle flowering in L.casa blanca .However, CEPA made a significantly negative correlation with ending flowering;2)The treatment of GA3 and CEPA both advanced the photosynthetic pigment in leaves of L.casa blanca ,and alleviated the downward trend of chlorophyll at the ending flowering time effectively,also it prolonged the photosynthesis time in leaves;3)The treatment of GA3 and CEPA both significantly promoted photosynthetic rate (P n ),conductance to H2 O (G s ), transpiration rate (T r )and stomatal limitation value (L s ),But there were a significantly negative correlation between intercellular CO2 concentration (C i ),transient energy utilization efficiency (LUE)and others.Analy-sis showed that,the treatment of GA3 and CEPA enhanced the activity of leaf cells,increased the intensity of photosynthesis in leaf;4)MDA content in leaves is lower than CK significantly under the treatment of GA3 and CEPA during the flowering phase,and the activity of SOD and CAT increased.At the same time,the treat-ment of GA3 and CEPA slowed down the downward trend of SOD activity in the middle and ending flowering time,relieved the ageing process of L.casa blanca ,and it reached the purpose of extending the time of blos-som of L.casa blanca .Comprehensive analysis shows that,the treatment of GA3 and CEPA advanced the pho-tosynthetic characteristics and antioxidant enzymes of L.casa blanca ,It made a significant influence in the blossom quality and flowering phase.Among them,the coordination treatment GA3 200 mg/L+CEPA 30 mg/L is the best in regulation on advancing flowering time and extending the days of blossom in L.casa blanca , followed by the single treatment CEPA 60 mg/L and GA3 200 mg/L.However,the adjust action decreased in high concentrations coordination treatment GA3 200 mg/L+CEPA 60 mg/L and GA3 300 mg/L+CEPA 30 mg/L.