CAJ | 학술논문

目的：探讨p16、p53及Ki－67在不同程度宫颈上皮内病变诊断中的临床病理意义。方法选取2012年1月至2014年6月期间成都市妇女儿童中心医院150例患有宫颈鳞癌、宫颈上皮内瘤变（ cervical intraepithelial neoplasia，CIN）以及正常宫颈的组织，用免疫组化SP法检测其p16、p53及Ki－67表达。结果 p16、p53、Ki－67在宫颈鳞癌组织中的阳性表达率分别为100％、46.67％、100％，在CINⅢ中的阳性表达率分别为95％、27.5％、100％，在CINⅡ中的阳性表达率分别为87.5％、22.5％、100％，在CINⅠ中的阳性表达率分别为63.5％、13.33％、100％。宫颈鳞癌组、CIN组（宫颈上皮内病变）中p16、p53及Ki－67的阳性表达率与对照组比较，差异均有统计学意义（ P＜0.05）。 p16、p53及Ki－67在CINⅠ、CINⅡ／Ⅲ及宫颈鳞癌的表达强度逐渐增加，且与CIN分级呈正相关。结论 p16、p53及Ki－67这3种相关蛋白作为辅助的诊断标记物对宫颈病变的诊治有参考价值。
목적：탐토p16、p53급Ki－67재불동정도궁경상피내병변진단중적림상병리의의。방법선취2012년1월지2014년6월기간성도시부녀인동중심의원150례환유궁경린암、궁경상피내류변（ cervical intraepithelial neoplasia，CIN）이급정상궁경적조직，용면역조화SP법검측기p16、p53급Ki－67표체。결과 p16、p53、Ki－67재궁경린암조직중적양성표체솔분별위100％、46.67％、100％，재CINⅢ중적양성표체솔분별위95％、27.5％、100％，재CINⅡ중적양성표체솔분별위87.5％、22.5％、100％，재CINⅠ중적양성표체솔분별위63.5％、13.33％、100％。궁경린암조、CIN조（궁경상피내병변）중p16、p53급Ki－67적양성표체솔여대조조비교，차이균유통계학의의（ P＜0.05）。 p16、p53급Ki－67재CINⅠ、CINⅡ／Ⅲ급궁경린암적표체강도축점증가，차여CIN분급정정상관。결론 p16、p53급Ki－67저3충상관단백작위보조적진단표기물대궁경병변적진치유삼고개치。
Objective investigation the pathological significance of p53, Ki-67 and p16 in diagnosis of cervical intraepithelial lesions.Methods Cervical tissues of 150 cases with squamous cell carcinoma of the uterine cervix, cervical intraepithelial neoplasia ( CIN) and normal cervical were extracted in Chengdu Women and Children’ s Central Hospital from Jan 2012 to Jun 2014, p16, p53 and Ki-67 were detected by immunohistochemical SP method.Results The expression positive rates of p16, p53 and Ki-67 in cervical squamous cell carcinoma group were 100 %, 46.67 %, 100 % respectively, those in CIN III were 95 %, 27.5 % and 100 %respectively, in CIN II were 87.5 %, 22.5 %, 100 %, in CIN I were 63.5 %, 13.33 %, 100 %.The differences of expression positive rates of p16, p53 and Ki-67 between cervical squamous cell carcinoma group, cervical intraepithelial neoplasia group and control group had statistical significant (P<0.05).The expressional intensity of p16, p53 and Ki-67p16 increased in CIN and cervical squamous cell carcinoma, which were positively associated with CIN degree.Conclusion The protein p16, P53 and Ki-67 have assisted reference value for cervical lesions as a diagnostic marker.