CAJ | 학술논문

目的：探讨白芍总苷（totalglucosidesofpaeony，TGP）对阿霉素肾病大鼠肾组织纤维化的影响及其机制。方法30只健康雄性SD大鼠尾静脉注射阿霉素制备模型，成模后随机分为模型对照组（ ADRN组）和白芍总苷干预组（ TGP组），同时设立正常对照组（ CON组）；干预8周后ELISA法检测血清Cr、BUN；尿蛋白定量法测24 h尿蛋白；Masson染色观察肾脏纤维化程度；RT－PCR法检测肾组织TLR4／NF－κB／TGF－β1 mRNA表达；免疫组化法检测肾组织TLR4／NF－κB／TGF－β1蛋白含量。结果与CON组相比， ADRN组大鼠血清Cr、BUN、24 h尿蛋白水平升高（ P＜0．01），肾组织纤维化程度加重（ P＜0．01）， TLR4／NF－κB／TGF－β1表达升高（P＜0．05）；与ADRN相比，TGP组大鼠Cr、BUN、24 h尿蛋白水平下降（P＜0．01），肾组织纤维化程度减轻（P＜0．01），TLR4／NF－κB／TGF－β1表达下降（ P＜0．05）。结论 TGP可以改善阿霉素肾病大鼠的肾脏功能，减轻病理损伤，其机制可能与下调TLR4／NF－κB／TGF－β1通路进而改善阿霉素大鼠肾纤维化有关。
목적：탐토백작총감（totalglucosidesofpaeony，TGP）대아매소신병대서신조직섬유화적영향급기궤제。방법30지건강웅성SD대서미정맥주사아매소제비모형，성모후수궤분위모형대조조（ ADRN조）화백작총감간예조（ TGP조），동시설립정상대조조（ CON조）；간예8주후ELISA법검측혈청Cr、BUN；뇨단백정량법측24 h뇨단백；Masson염색관찰신장섬유화정도；RT－PCR법검측신조직TLR4／NF－κB／TGF－β1 mRNA표체；면역조화법검측신조직TLR4／NF－κB／TGF－β1단백함량。결과여CON조상비， ADRN조대서혈청Cr、BUN、24 h뇨단백수평승고（ P＜0．01），신조직섬유화정도가중（ P＜0．01）， TLR4／NF－κB／TGF－β1표체승고（P＜0．05）；여ADRN상비，TGP조대서Cr、BUN、24 h뇨단백수평하강（P＜0．01），신조직섬유화정도감경（P＜0．01），TLR4／NF－κB／TGF－β1표체하강（ P＜0．05）。결론 TGP가이개선아매소신병대서적신장공능，감경병리손상，기궤제가능여하조TLR4／NF－κB／TGF－β1통로진이개선아매소대서신섬유화유관。
Objective To study effect and its possible mechanism of total glucosides of paeony ( TGP ) on adriamycin-induced nephropathy rats.Methods Thirty male SD rats were randomly divided into normal group ( CON) , adriamycin-induced nephropathy group ( ADRN) and TGP-treated ADRN group (TGP).The rat nephropathy model was established by adriamycin injection.At the end of the 8th week after treatment, ELISA was used to detect the level of Cr and BUN.The values of 24 urine protein was determined by urinary protein kit.Masson staining was used to observe the fibrosis.RT-PCR was used to detect the mRNA levels of TLR4/NF-κB/TGF-β1 .Immunohistochemical method was used to detect the content of TLR4/NF-κB/TGF-β1.ResuIts Compared with the CON group, the level of Cr, BUN, 24 urine protein of ADRN group rised(P<0.01), the degree of fibrosis of ADRN group were aggravated(P<0.01), and the expressions of TLR4/NF-κB/TGF-β1 of ADRN group increased significantly.However, compared with ADRN group, the(P<0.01) level of Cr, BUN, 24 urine protein of TGP-treated rats reduced(P<0.01), the degree of fibrosis of TGP-treated rats eased(P<0.05), and the expressions of TLR4/NF-κB/TGF-β1 of TGP-treated rats decreased significantly.ConcIusion TGP retards the process of fibrosis and reduces the pathological damage in adriamycin-induced rats by down-regulating the expression of TLR4/NF-κB/TGF-β1 signaling.