中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2015年
15期
733-736
,共4页
孙永林%李颖%李永文%吴志浩%范亚光%刘红雨%陈军
孫永林%李穎%李永文%吳誌浩%範亞光%劉紅雨%陳軍
손영림%리영%리영문%오지호%범아광%류홍우%진군
肿瘤坏死因子-α基因%单核苷酸多态性%原发性肺癌%小细胞肺癌%非小细胞肺癌
腫瘤壞死因子-α基因%單覈苷痠多態性%原髮性肺癌%小細胞肺癌%非小細胞肺癌
종류배사인자-α기인%단핵감산다태성%원발성폐암%소세포폐암%비소세포폐암
tumor necrosis factor-α gene%single nucleotide polymorphism%primary lung cancer%small cell lung cancer%non-small-cell lung cancer
目的：肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）基因启动子单核苷酸多态性与某些炎性疾病、肿瘤的发生有关。本研究就TNF-α-308G/A位点基因多态性与中国人群原发性肺癌易感性进行探讨。方法：应用高通量TaqMan-MGB探针技术对TNF-α-308G/A位点，即rs1800629位点进行基因分型，分析比较447例健康对照者和250例原发性肺癌患者的基因类型。采用SPSS 18.0软件对数据资料进行统计分析。结果：TNF-α-308G/A位点GG基因型在病例组和对照组中的频率分别为73.2%和90.8%。A/G基因型在病例组和对照组中频率分别为26.8%和8.7%。基因型A/G+AA频率分别为26.8%和9.2%，该位点多态性在病例组和对照组中的分布频率差异具有统计学意义（P<0.05）。此外，A/G基因型在男性、吸烟、小细胞肺癌或非小细胞肺癌患者相对于GG型均为危险因素，但在女性中无统计学意义（P>0.05）。结论：TNF-α-308G/A位点基因多态性与中国原发性肺癌发病易感性显著相关。
目的：腫瘤壞死因子-α（tumor necrosis factor-α，TNF-α）基因啟動子單覈苷痠多態性與某些炎性疾病、腫瘤的髮生有關。本研究就TNF-α-308G/A位點基因多態性與中國人群原髮性肺癌易感性進行探討。方法：應用高通量TaqMan-MGB探針技術對TNF-α-308G/A位點，即rs1800629位點進行基因分型，分析比較447例健康對照者和250例原髮性肺癌患者的基因類型。採用SPSS 18.0軟件對數據資料進行統計分析。結果：TNF-α-308G/A位點GG基因型在病例組和對照組中的頻率分彆為73.2%和90.8%。A/G基因型在病例組和對照組中頻率分彆為26.8%和8.7%。基因型A/G+AA頻率分彆為26.8%和9.2%，該位點多態性在病例組和對照組中的分佈頻率差異具有統計學意義（P<0.05）。此外，A/G基因型在男性、吸煙、小細胞肺癌或非小細胞肺癌患者相對于GG型均為危險因素，但在女性中無統計學意義（P>0.05）。結論：TNF-α-308G/A位點基因多態性與中國原髮性肺癌髮病易感性顯著相關。
목적：종류배사인자-α（tumor necrosis factor-α，TNF-α）기인계동자단핵감산다태성여모사염성질병、종류적발생유관。본연구취TNF-α-308G/A위점기인다태성여중국인군원발성폐암역감성진행탐토。방법：응용고통량TaqMan-MGB탐침기술대TNF-α-308G/A위점，즉rs1800629위점진행기인분형，분석비교447례건강대조자화250례원발성폐암환자적기인류형。채용SPSS 18.0연건대수거자료진행통계분석。결과：TNF-α-308G/A위점GG기인형재병례조화대조조중적빈솔분별위73.2%화90.8%。A/G기인형재병례조화대조조중빈솔분별위26.8%화8.7%。기인형A/G+AA빈솔분별위26.8%화9.2%，해위점다태성재병례조화대조조중적분포빈솔차이구유통계학의의（P<0.05）。차외，A/G기인형재남성、흡연、소세포폐암혹비소세포폐암환자상대우GG형균위위험인소，단재녀성중무통계학의의（P>0.05）。결론：TNF-α-308G/A위점기인다태성여중국원발성폐암발병역감성현저상관。
Objective:Previous studies suggested that the-308G/A allele in the tumor necrosis factor-α(TNF-α) gene promoter (-308G/A) may be a potential risk factor for inflammatory diseases and tumor progression. However, only a few studies have focused on the-308 polymorphism of TNF-αgene with primary lung cancer in Chinese population. This study aims to evaluate the role of TNF-α-308G/A single nucleotide polymorphism (SNP) and the risk of primary lung cancer in Chinese population. Methods:A total of 250 patients and 447 healthy individuals (control group) were involved in this study. Genotyping was performed using TaqMan technology. Results:The frequencies of (GG), (A/G), and (A/G+AA) genotypes of-308G/A SNP in TNF-αgene were 183 (73.2%), 67 (26.8%), and 67 (26.8%) in the patients, and 406 (90.8%), 39 (8.7%), and 41 (9.2%) in the control group, respectively. The distribution of poly-morphism frequencies in the case group and the control group showed a statistically significant difference for the Chinese population (P<0.05). Conclusion:Results indicated that TNF-αgene polymorphism at position-308G/A is associated with susceptibility to lung cancer in Chinese Han population.
