中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2015年
4期
510-512
,共3页
苏玉洁%冷玉芳%杨艳妮%王朋%郑贯峥%吕兴华
囌玉潔%冷玉芳%楊豔妮%王朋%鄭貫崢%呂興華
소옥길%랭옥방%양염니%왕붕%정관쟁%려흥화
乌头生物碱%肾%再灌注损伤
烏頭生物堿%腎%再灌註損傷
오두생물감%신%재관주손상
ACONITUM ALKALOIDS%Kidney%Reperfusion injury
目的 评价高乌甲素对大鼠肾缺血再灌注损伤的影响.方法 清洁级健康成年雄性Wistar大鼠36只,体重180~220 g,采用随机数字表法分为假手术组(S组)、肾缺血再灌注组(I/R组)和高乌甲素组(LA组),每组12只.I/R组和LA组采用游离左肾肾蒂,无损伤动脉夹夹闭左肾动脉45 min,松开左肾动脉夹后切除右肾的方法建立肾缺血再灌注损伤模型.LA组于再灌注前30min时腹腔注射高乌甲素4 mg/kg,S组和I/R组于同时点腹腔注射生理盐水2 ml.分别于再灌注5和24 h时采集下腔静脉血样,测定Cr和BUN的浓度;取肾组织,光镜下观察病理学结果,采用免疫组化法检测肾组织环氧合酶2(COX-2)和基质金属蛋白酶2(MMP-2)的表达.结果 与S组比较,I/R组和LA组再灌注5和24 h时血清Cr和BUN浓度升高,肾组织COX-2和MMP-2表达上调(P<0.05);与I/R组比较,LA组再灌注5和24 h时血清Cr和BUN浓度降低,肾组织COX-2和MMP-2表达下调(P<0.05),病理学损伤减轻.结论 高乌甲素可减轻大鼠肾缺血再灌注损伤,其机制与抑制COX-2和MMP-2的表达有关.
目的 評價高烏甲素對大鼠腎缺血再灌註損傷的影響.方法 清潔級健康成年雄性Wistar大鼠36隻,體重180~220 g,採用隨機數字錶法分為假手術組(S組)、腎缺血再灌註組(I/R組)和高烏甲素組(LA組),每組12隻.I/R組和LA組採用遊離左腎腎蒂,無損傷動脈夾夾閉左腎動脈45 min,鬆開左腎動脈夾後切除右腎的方法建立腎缺血再灌註損傷模型.LA組于再灌註前30min時腹腔註射高烏甲素4 mg/kg,S組和I/R組于同時點腹腔註射生理鹽水2 ml.分彆于再灌註5和24 h時採集下腔靜脈血樣,測定Cr和BUN的濃度;取腎組織,光鏡下觀察病理學結果,採用免疫組化法檢測腎組織環氧閤酶2(COX-2)和基質金屬蛋白酶2(MMP-2)的錶達.結果 與S組比較,I/R組和LA組再灌註5和24 h時血清Cr和BUN濃度升高,腎組織COX-2和MMP-2錶達上調(P<0.05);與I/R組比較,LA組再灌註5和24 h時血清Cr和BUN濃度降低,腎組織COX-2和MMP-2錶達下調(P<0.05),病理學損傷減輕.結論 高烏甲素可減輕大鼠腎缺血再灌註損傷,其機製與抑製COX-2和MMP-2的錶達有關.
목적 평개고오갑소대대서신결혈재관주손상적영향.방법 청길급건강성년웅성Wistar대서36지,체중180~220 g,채용수궤수자표법분위가수술조(S조)、신결혈재관주조(I/R조)화고오갑소조(LA조),매조12지.I/R조화LA조채용유리좌신신체,무손상동맥협협폐좌신동맥45 min,송개좌신동맥협후절제우신적방법건립신결혈재관주손상모형.LA조우재관주전30min시복강주사고오갑소4 mg/kg,S조화I/R조우동시점복강주사생리염수2 ml.분별우재관주5화24 h시채집하강정맥혈양,측정Cr화BUN적농도;취신조직,광경하관찰병이학결과,채용면역조화법검측신조직배양합매2(COX-2)화기질금속단백매2(MMP-2)적표체.결과 여S조비교,I/R조화LA조재관주5화24 h시혈청Cr화BUN농도승고,신조직COX-2화MMP-2표체상조(P<0.05);여I/R조비교,LA조재관주5화24 h시혈청Cr화BUN농도강저,신조직COX-2화MMP-2표체하조(P<0.05),병이학손상감경.결론 고오갑소가감경대서신결혈재관주손상,기궤제여억제COX-2화MMP-2적표체유관.
Objective To evaluate the effect of lappaconitine on renal ischemia-reperfusion (I/R) injury in mice.Methods Thirty-six male Wistar rats,weighing 180-220 g,were randomly divided into 3 groups (n=12 each) using a random number table:sham operation group (group S),group I/R and lappaconitine group (group LA).Renal I/R was induced by occlusion of the left renal pedicle for 45 min with atraumatic microclips followed by reperfusion,and the right kidney was removed after atraumatic microclips were released.At 30 min before reperfusion,lappaconitine 4 mg/kg was injected intraperitoneally in group LA,and normal saline 2 ml was given in S and I/R groups.In group S,the left renal pedicle was only isolated.At 5 and 24 h of reperfusion,blood samples were taken from the inferior vena cava for determination of serum creatinine (Cr) and blood urea nitrogen (BUN) concentrations,and kidney specimens were obtained for histopathologic examination (with light microscope) and for determination of the expression of cyclooxygenase-2 (COX-2) and matrix metalloproteinase-2 (MMP-2) in renal tissues (by immunohistochemistry).Results Compared with group S,the serum Cr and BUN concentrations were significantly increased,and the expression of COX-2 and MMP-2 in renal tissues was up-regulated at 5 and 24 h of reperfusion in I/R and LA groups.Compared with group I/R,the serum Cr and BUN concentrations were significantly decreased,the expression of COX-2 and MMP-2 in renal tissues was down-regulated at 5 and 24 h of reperfusion and histopathologic changes were reduced in group LA.Conclusion Lappaconitine can attenuate renal I/R injury through inhibiting the expression of COX-2 and MMP-2 in rats.