中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2015年
8期
747-750
,共4页
曹明燕%胡仕敏%张子阳%杨亦彬
曹明燕%鬍仕敏%張子暘%楊亦彬
조명연%호사민%장자양%양역빈
糖尿病肾病%促血管生成素-1%腺病毒
糖尿病腎病%促血管生成素-1%腺病毒
당뇨병신병%촉혈관생성소-1%선병독
Diabetic nephropathy(DN)%Angiopoietin-1(Ang-1)%Adenovirus
目的：探讨血管生成素‐1（Ang‐1）对糖尿病大鼠肾脏病变的影响。方法96只雄性SD大鼠随机分为正常对照（NC）组、DN组、Ang‐1载体（AV ）组和空载体（BV ）组。STZ诱导DN模型，8周后尾静脉注射A ng‐1‐腺病毒载体或腺病毒空载体。检测不同时点血糖、尿蛋白及肾脏病理；采用免疫组织化学、荧光和RT‐PCR检测肾组织Ang‐1及其受体络氨酸激酶‐2（Tie‐2）蛋白和mRNA。结果 AV组24 hUAlb[8周（52.33±15.67）mg／24 h ，28周（40.50±7.42）mg／24 h]及血糖[8周（26.28±0.81） mmol／L ，28周（17.48±1.14）mmol／L]降低（P＜0.05）；肾脏病理变化减轻，肾小球面积未减小（P＞0.05）；肾组织 Ang‐1、Tie‐2 mRNA[8周（76.55±13.21），28周（90.47±5.04）]及蛋白[8周（140.85±8.45），28周（150.84±10.48）]升高（P＜0.05）。结论外源性Ang‐1可上调DN大鼠肾组织 Ang‐1和T ie‐2的表达，缓解D N。
目的：探討血管生成素‐1（Ang‐1）對糖尿病大鼠腎髒病變的影響。方法96隻雄性SD大鼠隨機分為正常對照（NC）組、DN組、Ang‐1載體（AV ）組和空載體（BV ）組。STZ誘導DN模型，8週後尾靜脈註射A ng‐1‐腺病毒載體或腺病毒空載體。檢測不同時點血糖、尿蛋白及腎髒病理；採用免疫組織化學、熒光和RT‐PCR檢測腎組織Ang‐1及其受體絡氨痠激酶‐2（Tie‐2）蛋白和mRNA。結果 AV組24 hUAlb[8週（52.33±15.67）mg／24 h ，28週（40.50±7.42）mg／24 h]及血糖[8週（26.28±0.81） mmol／L ，28週（17.48±1.14）mmol／L]降低（P＜0.05）；腎髒病理變化減輕，腎小毬麵積未減小（P＞0.05）；腎組織 Ang‐1、Tie‐2 mRNA[8週（76.55±13.21），28週（90.47±5.04）]及蛋白[8週（140.85±8.45），28週（150.84±10.48）]升高（P＜0.05）。結論外源性Ang‐1可上調DN大鼠腎組織 Ang‐1和T ie‐2的錶達，緩解D N。
목적：탐토혈관생성소‐1（Ang‐1）대당뇨병대서신장병변적영향。방법96지웅성SD대서수궤분위정상대조（NC）조、DN조、Ang‐1재체（AV ）조화공재체（BV ）조。STZ유도DN모형，8주후미정맥주사A ng‐1‐선병독재체혹선병독공재체。검측불동시점혈당、뇨단백급신장병리；채용면역조직화학、형광화RT‐PCR검측신조직Ang‐1급기수체락안산격매‐2（Tie‐2）단백화mRNA。결과 AV조24 hUAlb[8주（52.33±15.67）mg／24 h ，28주（40.50±7.42）mg／24 h]급혈당[8주（26.28±0.81） mmol／L ，28주（17.48±1.14）mmol／L]강저（P＜0.05）；신장병리변화감경，신소구면적미감소（P＞0.05）；신조직 Ang‐1、Tie‐2 mRNA[8주（76.55±13.21），28주（90.47±5.04）]급단백[8주（140.85±8.45），28주（150.84±10.48）]승고（P＜0.05）。결론외원성Ang‐1가상조DN대서신조직 Ang‐1화T ie‐2적표체，완해D N。
Objective To investigate the effect of recombinant adenovirus angiopoietin‐1(Ang‐1) on renal lesions in diabetic rats. Methods A total of 96 male SD rats were randomly divided into 4 groups :normal control (NC) group ,diabetic nephropathy (DN) group ,Ang‐1‐vector (AV) group and blank vector (BV) group. The DN model was induced by injection of streptozotocina. Ang‐1‐vector and blank vector were injected by tail intravenous injection 8 weeks later. The blood glucose ,urine protein ,and renal pathology of different time points were detected. Ang‐1 ,Tie‐2 protein and its mRNA in renal tissue were tested by RT‐PCR ,immunochemistry and immunofluorescence respectively. Results The 24 hUAlb[8 weeks(52.33 ± 15.67)mg/24 h ,28 weeks(40.50 ± 7.42)mg/24 h] and blood glucose [8 weeks(26.28 ± 0.81) mmol/L ,28 weeks (17.48 ± 1.14 ) mmol/L ] were decreased significantly ( P < 0.05 ) ,renal pathological change was alleviated ,and the decrease of glomerular area in Ang‐1 treated group was not significant (P>0.05). The expressional of Ang‐1 and Tie‐2 mRNA [8 weeks (76.55 ± 13.21) ,28 weeks (90.47 ± 5.04)]and protein in renal tissue [8 weeks (140.85 ± 8.45) ,28 weeks(150.84 ± 10.48)]were increased significantly( P<0.05). Conclusion Exogenous Ang‐1 can upregulate the expression of Ang‐1 and Tie‐2 in renal tissue ,and relieve renal lesions in diabetic rats.
