CAJ | 학술논문

目的：通过研究富含半胱氨酸的酸性分泌蛋白（ SPARC）在喉癌及下咽癌的蛋白表达与相关功能，探讨SPARC对头颈鳞癌发展所起的作用。方法：免疫组织化学染色检测SPARC在51例肿瘤组织中的表达，观察外源性SPARC对体外细胞系生长及迁移的影响。结果：免疫组化染色显示SPARC在46.3％（19/41）喉癌及60.0％（6/10）下咽癌的间质组织表达下调，外源性SPARC抑制头颈鳞癌细胞株的增殖及迁移。结论：肿瘤间质SPARC表达下调促进了喉癌及下咽癌细胞的增殖。
목적：통과연구부함반광안산적산성분비단백（ SPARC）재후암급하인암적단백표체여상관공능，탐토SPARC대두경린암발전소기적작용。방법：면역조직화학염색검측SPARC재51례종류조직중적표체，관찰외원성SPARC대체외세포계생장급천이적영향。결과：면역조화염색현시SPARC재46.3％（19/41）후암급60.0％（6/10）하인암적간질조직표체하조，외원성SPARC억제두경린암세포주적증식급천이。결론：종류간질SPARC표체하조촉진료후암급하인암세포적증식。
Objective:To explore the contribution of SPARC for head and neck squamous carcinoma,investigate protein expression and function of SPARC in laryngeal and hypopharyngeal carcinoma. Methods:Immunohistochemi-cal staining was used to detect the expression of SPARC in primary tumor,recombinant human SPARC was used to de-termine the effect of exogenous SPARC on growth and migration of tumor cell lines. Results:Immunohistochemical staining showed that stromal SPARC was down-regulated in 46. 3%(19/41)of laryngeal carcinoma and 60. 0%(6/10)of hypopharyngeal carcinoma. Exogenous SPARC suppressed the growth and migration of tumor cell lines. Conclu-sion:Down-regulated expression of stromal SPARC promotes the proliferation of laryngeal and hypopharyngeal carci-noma cells.