中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
31期
2532-2536
,共5页
徐敏鸥%陈哲%周泽著%郑月焕%袁野%张庆华%曹鹏
徐敏鷗%陳哲%週澤著%鄭月煥%袁野%張慶華%曹鵬
서민구%진철%주택저%정월환%원야%장경화%조붕
椎间盘%腰椎%基因表达型%Modic病变
椎間盤%腰椎%基因錶達型%Modic病變
추간반%요추%기인표체형%Modic병변
Intervertebral disk%Lumbar vertebrae%Gene expression profiling%Modic changes
目的 筛查Modic病变的相关差异表达基因.方法 2014年8-12月获取上海交通大学医学院附属瑞金医院骨科与临海市第二人民医院骨科5例伴Modic病变的终板和软骨下骨组织样本和5例不伴Modic病变的对照组织样本,采用基因芯片对组织标本进行基因表达谱分析.筛查小的差异基因进行GO (Gene Ontology)和KEGG功能分析.最后采用聚合酶链反应(PCR)的方法对筛查出来的差异基因进行定量验证.结果 共筛选出263个差异基因,其中107个为上调基因,156个为下调基因(P＜0.05,Fold Change＞2).这些差异基因主要涉及趋化作用、细胞运动等生物学过程.其中,2个上调基因(CXCL14、KCNMA1)与4个下调基因(MARCKS、ZEB2、PSMF1 、CNN2)进行了定量PCR验证,其表达趋势与基因芯片检测结果一致.结论 Modic病变的终板和软骨下骨组织与非Modic病变的组织存在显著的基因表达差异.Modic病变可能是一种多基因参与、多基因调控的病变.
目的 篩查Modic病變的相關差異錶達基因.方法 2014年8-12月穫取上海交通大學醫學院附屬瑞金醫院骨科與臨海市第二人民醫院骨科5例伴Modic病變的終闆和軟骨下骨組織樣本和5例不伴Modic病變的對照組織樣本,採用基因芯片對組織標本進行基因錶達譜分析.篩查小的差異基因進行GO (Gene Ontology)和KEGG功能分析.最後採用聚閤酶鏈反應(PCR)的方法對篩查齣來的差異基因進行定量驗證.結果 共篩選齣263箇差異基因,其中107箇為上調基因,156箇為下調基因(P＜0.05,Fold Change＞2).這些差異基因主要涉及趨化作用、細胞運動等生物學過程.其中,2箇上調基因(CXCL14、KCNMA1)與4箇下調基因(MARCKS、ZEB2、PSMF1 、CNN2)進行瞭定量PCR驗證,其錶達趨勢與基因芯片檢測結果一緻.結論 Modic病變的終闆和軟骨下骨組織與非Modic病變的組織存在顯著的基因錶達差異.Modic病變可能是一種多基因參與、多基因調控的病變.
목적 사사Modic병변적상관차이표체기인.방법 2014년8-12월획취상해교통대학의학원부속서금의원골과여림해시제이인민의원골과5례반Modic병변적종판화연골하골조직양본화5례불반Modic병변적대조조직양본,채용기인심편대조직표본진행기인표체보분석.사사소적차이기인진행GO (Gene Ontology)화KEGG공능분석.최후채용취합매련반응(PCR)적방법대사사출래적차이기인진행정량험증.결과 공사선출263개차이기인,기중107개위상조기인,156개위하조기인(P＜0.05,Fold Change＞2).저사차이기인주요섭급추화작용、세포운동등생물학과정.기중,2개상조기인(CXCL14、KCNMA1)여4개하조기인(MARCKS、ZEB2、PSMF1 、CNN2)진행료정량PCR험증,기표체추세여기인심편검측결과일치.결론 Modic병변적종판화연골하골조직여비Modic병변적조직존재현저적기인표체차이.Modic병변가능시일충다기인삼여、다기인조공적병변.
Objective To identify differentially expressed genes in Modic changes by gene microarray method.Methods From August 2014 to December 2014,gene expression profiling analysis of 5 lumbar endplates with Modic changes and 5 control specimens without Modic changes were performed in Department of Orthopaedics,Zheng Linhai Second People's Hospital and Department of Orthopaedics,Shanghai Jiaotong University School of Medicine.The functional analysis (Gene Ontology and KEGG) of deregulated genes was carried out.The qRT-PCR analysis was performed to validate differential expression genes.Results Of 263 significantly differential expression genes (P ＜ 0.05,Fold Change ＞ 2),107 were over-expressed and 156 under-expressed genes.Those deregulated genes were mainly involved in chemotaxis and cell motion.The qRT-PCR analysis of 2 up-regulated genes (CXCL14,KCNMAl) and 4 underregulated genes (MARCKS,ZEB2,PSMFl,and CNN2) mRNA expression levels validated the results from the microarray analysis.Conclusions There are differentially expressed genes between lumbar endplate with Modic changes and without Modic changes.Modic changes may be multiple genes involved and regulated pathological changes.
