中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
31期
2560-2564
,共5页
陆爽%程明亮%杨德猛%刘洋%关丽%吴君
陸爽%程明亮%楊德猛%劉洋%關麗%吳君
륙상%정명량%양덕맹%류양%관려%오군
细胞凋亡%肝硬化%蓝莓%肝细胞
細胞凋亡%肝硬化%藍莓%肝細胞
세포조망%간경화%람매%간세포
Apoptosis%Liver cirrhosis%Bluberry%Hepatocytes
目的 探讨蓝莓对大鼠肝星状细胞(HSC-T6)凋亡及Bcl-2和Bax表达的影响.方法 血清药理学方法制备大鼠10%蓝莓低、中、高剂量血清、复方鳖甲软肝片血清及生理盐水血清.传代培养的HSC-T6分为生理盐水血清组、蓝莓低剂量血清组、蓝莓中剂量血清组、蓝莓高剂量血清组及复方鳖甲软肝片血清组,将上述血清分别加入各组HSC-T6,培养72 h后采用Annexin V/PI检测各组HSC-T6的凋亡,Western印迹及免疫细胞化学检测各组HSC-T6 Bax及Bcl-2蛋白的表达.结果 与生理盐水血清组比较,蓝莓低、中、高剂量血清组HSC-T6 Bax蛋白表达差异无统计学意义;蓝莓高剂量血清组HSC-T6早期及总凋亡率明显高于生理盐水血清组(5.55%±0.98%比2.53%±0.46%,7.01%±1.05%比2.96%±0.81%,均P<0.05);Bcl-2蛋白表达明显低于生理盐水血清组(A值,82±35比51±13,P<0.05);Bcl-2/Bax比值明显低于生理盐水血清组(0.26±0.02比0.46±0.03,P<0.05);蓝莓低、中剂量血清组HSC-T6早期及总凋亡率、Bcl-2的表达及Bcl-2/Bax的比值与生理盐水血清组比较差异均无统计学意义.结论 蓝莓可通过下调Bcl-2、降低Bcl-2/Bax的比值诱导HSC-T6的凋亡,从而对大鼠肝纤维化有潜在的干预作用.
目的 探討藍莓對大鼠肝星狀細胞(HSC-T6)凋亡及Bcl-2和Bax錶達的影響.方法 血清藥理學方法製備大鼠10%藍莓低、中、高劑量血清、複方鱉甲軟肝片血清及生理鹽水血清.傳代培養的HSC-T6分為生理鹽水血清組、藍莓低劑量血清組、藍莓中劑量血清組、藍莓高劑量血清組及複方鱉甲軟肝片血清組,將上述血清分彆加入各組HSC-T6,培養72 h後採用Annexin V/PI檢測各組HSC-T6的凋亡,Western印跡及免疫細胞化學檢測各組HSC-T6 Bax及Bcl-2蛋白的錶達.結果 與生理鹽水血清組比較,藍莓低、中、高劑量血清組HSC-T6 Bax蛋白錶達差異無統計學意義;藍莓高劑量血清組HSC-T6早期及總凋亡率明顯高于生理鹽水血清組(5.55%±0.98%比2.53%±0.46%,7.01%±1.05%比2.96%±0.81%,均P<0.05);Bcl-2蛋白錶達明顯低于生理鹽水血清組(A值,82±35比51±13,P<0.05);Bcl-2/Bax比值明顯低于生理鹽水血清組(0.26±0.02比0.46±0.03,P<0.05);藍莓低、中劑量血清組HSC-T6早期及總凋亡率、Bcl-2的錶達及Bcl-2/Bax的比值與生理鹽水血清組比較差異均無統計學意義.結論 藍莓可通過下調Bcl-2、降低Bcl-2/Bax的比值誘導HSC-T6的凋亡,從而對大鼠肝纖維化有潛在的榦預作用.
목적 탐토람매대대서간성상세포(HSC-T6)조망급Bcl-2화Bax표체적영향.방법 혈청약이학방법제비대서10%람매저、중、고제량혈청、복방별갑연간편혈청급생리염수혈청.전대배양적HSC-T6분위생리염수혈청조、람매저제량혈청조、람매중제량혈청조、람매고제량혈청조급복방별갑연간편혈청조,장상술혈청분별가입각조HSC-T6,배양72 h후채용Annexin V/PI검측각조HSC-T6적조망,Western인적급면역세포화학검측각조HSC-T6 Bax급Bcl-2단백적표체.결과 여생리염수혈청조비교,람매저、중、고제량혈청조HSC-T6 Bax단백표체차이무통계학의의;람매고제량혈청조HSC-T6조기급총조망솔명현고우생리염수혈청조(5.55%±0.98%비2.53%±0.46%,7.01%±1.05%비2.96%±0.81%,균P<0.05);Bcl-2단백표체명현저우생리염수혈청조(A치,82±35비51±13,P<0.05);Bcl-2/Bax비치명현저우생리염수혈청조(0.26±0.02비0.46±0.03,P<0.05);람매저、중제량혈청조HSC-T6조기급총조망솔、Bcl-2적표체급Bcl-2/Bax적비치여생리염수혈청조비교차이균무통계학의의.결론 람매가통과하조Bcl-2、강저Bcl-2/Bax적비치유도HSC-T6적조망,종이대대서간섬유화유잠재적간예작용.
Objective To investigate the effects of blneberry on the apoptosis,expression of Bcl-2 and Bax in rat hepatic stellate cell (HSC-T6).Methods 10% blueberry serum at low,middle and high dose,10% Fu-Fang-Bie-Jia-Ruan-Gan tablet serum and 10% saline serum were prepared by method of serum pharmacology.Subcultured HSC-T6 was divided into saline serum control group,blueberry serum at low,middle,high dose and Fu-Fang-Bie-Jia-Ruan-Gan tablet serum group,and then was respectively incubated at different dose of 10% blueberry serum,10% Fu-Fang-Bie-Jia-Ruan-Gan tablet serum and 10% saline serum for 72 hours.Apoptosis of HSC-T6 was detected using flow cytometry with annexin V FITC/PI double staining.The expression of Bcl-2 and Bax in HSC-T6 were examined using immunocytochemistry and Western blotting,respectively.Result There was no significant difference for HSC-T6 Bax protein expression in the low,middle and high dose blueberry serum groups,compared with saline serum control group,respectively.In the high-dose blueberry serum group HSC-T6 early and total apoptosis rate incrcascd significantly compared with the saline serum control group (5.55% ± 0.98% vs 2.53% ± 0.46%,7.01%±1.05% vs 2.96% ± 0.81%,both P <0.05);Bcl-2 protein expression was significantly decreased (A value,82 ± 35 vs 51 ± 13,P < 0.05);Bcl-2/Bax ratio was significantly decreased (0.26 ± 0.02 vs 0.46 ± 0.03,P < 0.05);HSC-T6 early and total apoptosis rate,Bcl-2 expression and Bcl-2/Bax ratio in the low and the middle dose blueberry serum group showed no significant difference with the saline serum control group.Conclusion Blueberry can induce HSC-T6 apoptosis by down-regulating Bcl-2 expression and decreasing the ratio of Bcl-2/Bax in HSC-T6 cells,so it may have potential interference effects on hepatic fibrosis.