CAJ | 학술논문

PIN2蛋白作为生长素外排蛋白，在植物根的向地性生长中起重要作用。免疫定位法是一种可以将蛋白在植物细胞中快速准确定位的方法。本研究利用改良的免疫荧光定位法，对 PIN2蛋白在水稻根尖细胞中的定位进行了观察，并与前人的两种方法进行比较。结果表明，与已有的方案比较，改良免疫荧光定位法可使 PIN2蛋白在水稻根尖细胞中的位置快速准确地被观察到，且简化了操作步骤，操作方便快速，可广泛应用于植物其他蛋白的定位研究。
PIN2단백작위생장소외배단백，재식물근적향지성생장중기중요작용。면역정위법시일충가이장단백재식물세포중쾌속준학정위적방법。본연구이용개량적면역형광정위법，대 PIN2단백재수도근첨세포중적정위진행료관찰，병여전인적량충방법진행비교。결과표명，여이유적방안비교，개량면역형광정위법가사 PIN2단백재수도근첨세포중적위치쾌속준학지피관찰도，차간화료조작보취，조작방편쾌속，가엄범응용우식물기타단백적정위연구。
PIN2 as an auxin efflux protein plays important roles in geotropic growth of plant roots.Immu-nolocalization technique is a method which allows rapid and reliable in situ localization of proteins in plant cells.In this paper,the improved immunolocalization technique was used to localize the PIN2 in root tip cells of rice,and its effect was compared to the two existing methods.The results showed that the location of PIN2 could be more quickly and accurately determined in root tip cells of rice by the improved method;and the im-proved method simplified the operation procedures and was more convenient,so it could be used for the locali-zation researches of other plant proteins in the future.