CAJ | 학술논문

为给水生植物芡实（Euryale ferox）生物肥料的研制提供优良的菌株资源，采用保绿法和萝卜子叶增重法从芡实根际土壤筛选出2株作用效果较好的细菌 Q6－2和 Q67，用高效液相色谱法（High Performance Liquid Chromatography，HPLC）定量分析菌株产植物激素和有机酸的能力；通过盆栽试验研究2株细菌对苗期芡实的促生作用；结合生理生化试验和16S rDNA 序列分析对2株细菌进行鉴定。结果显示，2株细菌均能够产生玉米素、激动素等植物激素和少量有机酸，Q6－2还能够产生吲哚乙酸；接种 Q6－2后，芡实幼苗茎长、叶片数、最大叶面积、生物量（干重）分别比对照高出18．85％、40．67％、76．28％和37．50％，接种 Q67的处理各项指标分别比对照高出27．47％、42．81％、96．05％和42．97％，差异显著；接种 Q6－2和 Q67后，叶绿素含量分别高出对照43．27％和51．46％，根系活力则分别高出5．41％和7．05％，差异显著。经鉴定，Q6－2和 Q67均为地衣芽孢杆菌，GenBank 登录号分别为 KP686129和 KP686133。2株细菌有效促进了芡实幼苗生长发育水平，在微生物肥料的生产中具有广阔的应用前景。
위급수생식물검실（Euryale ferox）생물비료적연제제공우량적균주자원，채용보록법화라복자협증중법종검실근제토양사선출2주작용효과교호적세균 Q6－2화 Q67，용고효액상색보법（High Performance Liquid Chromatography，HPLC）정량분석균주산식물격소화유궤산적능력；통과분재시험연구2주세균대묘기검실적촉생작용；결합생리생화시험화16S rDNA 서렬분석대2주세균진행감정。결과현시，2주세균균능구산생옥미소、격동소등식물격소화소량유궤산，Q6－2환능구산생신타을산；접충 Q6－2후，검실유묘경장、협편수、최대협면적、생물량（간중）분별비대조고출18．85％、40．67％、76．28％화37．50％，접충 Q67적처리각항지표분별비대조고출27．47％、42．81％、96．05％화42．97％，차이현저；접충 Q6－2화 Q67후，협록소함량분별고출대조43．27％화51．46％，근계활력칙분별고출5．41％화7．05％，차이현저。경감정，Q6－2화 Q67균위지의아포간균，GenBank 등록호분별위 KP686129화 KP686133。2주세균유효촉진료검실유묘생장발육수평，재미생물비료적생산중구유엄활적응용전경。
This study aims to provide excellent bacterial strain resources for the production of Euryale fer-ox bio -fertilizer.Two strains with relatively higher performance named Q6 -2 and Q67 were screened from the rhizosphere soil of Euryale ferox using the methods of remaining green and radish cotyledon bioassay;their production capability of plant hormones and organic acids were quantitatively analyzed by HPLC;their promo-tion effects on the growth of Euryale ferox at seedling stage were investigated by pot experiments;also,they were identified by physiological and biochemical experiments and 16S rDNA sequence analysis.The results in-dicated that the two strains were both able to produce zeatin,kinetin and small amount of organic acid.Q6 -2 was also able to produce indoleacetic acid (IAA).After inoculated by Q6 -2,the growth parameters of Eury-ale ferox seedlings,such as stem length,number of leaves,maximum leaf area and biomass (dry weight), significantly increased by 18.85%,40.67%,76.28% and 37.50% respectively compared to the control;while inoculated by Q67,the above mentioned indexes significantly increased by 27.47%,42.81%, 96.05% and 42.97% respectively.After inoculated by Q6 -2 and Q67,the chlorophyll content increased by 43.27% and 51.46% respectively,and the root activity increased by 5.41% and 7.05% respectively,show-ing significant differences compared to the control.Q6 -2 and Q67 were both identified as Bacillus lichenifor-mis,whose GenBank accession number were KP686129 and KP686133 respectively.In conclusion,the two strains were able to promote the growth and development of Euryale ferox seedlings efficiently,showing great application prospect in the production of microbial fertilizer.