CAJ | 학술논문

背景：干细胞移植后需要对体内的存活、分布、迁徙、增殖及分化进行连续监测，从而评估其疗效和安全性。目的：分析近10年国内外干细胞标记及MRI示踪研究的文献学特点。 　　方法：由第一作者用计算机检索中国期刊全文数据库(CNKI)，及国际数据库Web of Science数据库，2005年1月至2014年12月关于干细胞磁共振成像示踪研究的文章，在标题和摘要中以“干细胞(stem cel )，磁共振成像(magnetic resonance imaging)，示踪技术(tracing technique)”为检索词进行检索，并对文献进行深入分析。 　　结果与结论：目前，干细胞治疗、器官移植、细菌感染、基因表达、新药开发等众多领域已经广泛应用示踪技术。干细胞的示踪技术主要有同位素示踪法、抗原标记法、荧光蛋白标记法、荧光染料标记法、核磁共振对比增强剂标记法、Lac-Z基因标记法、Y染色体标记法。近年来，随着分子影像学的发展，磁共振成像示踪技术在评估干细胞移植的疗效和安全性中应用越来越多。
배경：간세포이식후수요대체내적존활、분포、천사、증식급분화진행련속감측，종이평고기료효화안전성。목적：분석근10년국내외간세포표기급MRI시종연구적문헌학특점。 　　방법：유제일작자용계산궤검색중국기간전문수거고(CNKI)，급국제수거고Web of Science수거고，2005년1월지2014년12월관우간세포자공진성상시종연구적문장，재표제화적요중이“간세포(stem cel )，자공진성상(magnetic resonance imaging)，시종기술(tracing technique)”위검색사진행검색，병대문헌진행심입분석。 　　결과여결론：목전，간세포치료、기관이식、세균감염、기인표체、신약개발등음다영역이경엄범응용시종기술。간세포적시종기술주요유동위소시종법、항원표기법、형광단백표기법、형광염료표기법、핵자공진대비증강제표기법、Lac-Z기인표기법、Y염색체표기법。근년래，수착분자영상학적발전，자공진성상시종기술재평고간세포이식적료효화안전성중응용월래월다。
BACKGROUND:Continuous monitoring is required in the survival, distribution, migration, proliferation and differentiation of transplanted stem cel s in vivo thereby to assess its efficacy and safety. OBJECTIVE:To analyze the characteristics of literature addressing stem cel markers and MRI tracer in the past 10 years. METHODS:The first author retrieved CNKI and Web of Science by computer for articles about MRI tracing of stem cel s published from January 2005 to December 2014, using the keywords of“stem cel s, magnetic resonance imaging, tracing technique”that appeared in the title and abstract. Then, the retrieved articles were further analyzed. RESULTS AND CONCLUSION:Currently, tracing technique has been widely used in stem cel therapy, organ transplantation, bacterial infection, gene expression and new drug development. Stem cel tracing technologies include isotope tracing, antigenic labeling, fluorescence protein labeling, fluorescent dye labeling, nuclear magnetic resonance contrast enhancer labeling, Lac-Z gene labeling, Y chromosome labeling. Recently, with the development of molecular imaging, MRI tracing techniques have been increasingly used to assess the efficacy and safety of stem cel transplantation.