CAJ | 학술논문

背景：研究证实骨髓间充质干细胞对于脑缺血再灌注损伤有积极的治疗作用，但是经尾静脉给予骨髓间充质干细胞条件培养基的研究报道较少。 　　目的：观察经尾静脉注射骨髓间充质干细胞条件培养基对大鼠脑缺血再灌注损伤后神经功能恢复的影响。 　　方法：体外分离培养大鼠骨髓间充质干细胞，第2、3代骨髓间充质干细胞培养融合达90%时弃培养基，DMEM培养基无血清培养18 h，收集的培养液即为骨髓间充质干细胞条件培养基。将36只右侧大脑中动脉缺血再灌注模型大鼠随机分为3组：对照组，单纯培养基对照组和条件培养基组，分别于术后2，24，48 h经尾静脉给予生理盐水、DMEM培养基、条件培养基(10 mL/kg)。 　　结果与结论：术后2 h各组大鼠神经功能缺损评分差异无显著性意义(P>0.05)。术后第1，3，5天对照组与单纯培养基组大鼠神经功能缺损评分差异无显著性意义，条件培养基组大鼠神经功能评分明显低于对照组和单纯培养基组，差异有显著性意义(P<0.05)。术后5 d条件培养基组较对照组、单纯培养基组脑组织含水量显著减少(P <0.05)，对照组和单纯培养基组相比差异无显著性意义(P >0.05)。结果说明尾静脉注射骨髓间充质干细胞条件培养基可以减轻大鼠脑缺血再灌注损伤后脑水肿程度，改善神经功能。
배경：연구증실골수간충질간세포대우뇌결혈재관주손상유적겁적치료작용，단시경미정맥급여골수간충질간세포조건배양기적연구보도교소。 　　목적：관찰경미정맥주사골수간충질간세포조건배양기대대서뇌결혈재관주손상후신경공능회복적영향。 　　방법：체외분리배양대서골수간충질간세포，제2、3대골수간충질간세포배양융합체90%시기배양기，DMEM배양기무혈청배양18 h，수집적배양액즉위골수간충질간세포조건배양기。장36지우측대뇌중동맥결혈재관주모형대서수궤분위3조：대조조，단순배양기대조조화조건배양기조，분별우술후2，24，48 h경미정맥급여생리염수、DMEM배양기、조건배양기(10 mL/kg)。 　　결과여결론：술후2 h각조대서신경공능결손평분차이무현저성의의(P>0.05)。술후제1，3，5천대조조여단순배양기조대서신경공능결손평분차이무현저성의의，조건배양기조대서신경공능평분명현저우대조조화단순배양기조，차이유현저성의의(P<0.05)。술후5 d조건배양기조교대조조、단순배양기조뇌조직함수량현저감소(P <0.05)，대조조화단순배양기조상비차이무현저성의의(P >0.05)。결과설명미정맥주사골수간충질간세포조건배양기가이감경대서뇌결혈재관주손상후뇌수종정도，개선신경공능。
BACKGROUND:Large numbers of experimental data have confirmed that bone marrow mesenchymal stem cel s have a positive therapeutic effect on cerebral ischemia-reperfusion injury, but there are few reports about intravenous administration of bone marrow mesenchymal stem cel conditioned medium in the treatment of stroke. OBJECTIVE:To investigate the effects of the conditioned medium of rat bone marrow mesenchymal stem cel s on the recovery of neurological function in rats after cerebral ischemia-reperfusion injury. METHODS:The bone marrow mesenchymal stem cel s were isolated from rat bone marrow. When cel s at passage 2 or 3 reached 90%confluence, the original culture medium was removed. Then the cel s were cultured in serum-free DMEM for 18 hours. After that, the culture solution was col ected as the conditioned medium of rat bone marrow mesenchymal stem cel s. Adult rats were subjected to 2 hours of right middle cerebral artery occlusion. Ischemia-reperfusion injury rats were randomly assigned to three groups:control group, simple culture medium group and conditioned medium group, and respectively given injection of normal saline, DMEM, conditioned medium (10 mL/kg) via the tail vein at 2, 24, 48 hours after operation. RESULTS AND CONCLUSION:There was no difference in the behavioral tests among the three groups at postoperative 2 hours (P>0.05). Compared with the control and simple culture medium group, neurological impairment was significantly improved in the conditioned medium group at postoperative 1, 3, 5 days (P<0.05), but there was no significant difference between the control and simple culture medium groups. At postoperative 5 days, brain edema was significantly eased in the conditioned medium group in comparison to the control and simple culture medium groups (P<0.05), and there was also no difference between the latter two groups (P>0.05). These results suggest that rat bone marrow mesenchymal stem cel s-conditioned medium via intravenous administration can significantly ease brain edema and improve the neurologic function after cerebral ischemia-reperfusion injury.