CAJ | 학술논문

背景：单一支架材料在不同程度不同范围内存在一些缺点，因此近年来研究者选择复合支架材料，将两种或多种具有一定互补特性的生物材料按一定方式与比例复合，构造出新型复合材料。 　　目的：探讨可注射型geneX人工骨复合骨髓间充质干细胞/转化生长因子β2的组织相容性及体外成骨诱导分化效果。 　　方法：将可注射型geneX人工骨与兔骨髓间充质干细胞复合培养5 d，电镜观察人工骨的组织相容性。取第3代成骨诱导培养的兔骨髓间充质干细胞分3组培养，单纯细胞组仅加入成骨诱导分化培养基，细胞支架组加入geneX人工骨与成骨诱导分化培养基，联合组加入geneX人工骨、转化生长因子β2与成骨诱导分化培养基，培养7，14，21 d进行细胞形态学观察、碱性磷酸酶活性检测、MTT检测、甲基百里香酚蓝检测及茜素红染色观察。 　　结果与结论：骨髓间充质干细胞在可注射型geneX人工骨表面贴壁生长，呈成纤维细胞形态，细胞基质分泌旺盛。联合组细胞增殖、成骨诱导活性强于单纯细胞组、细胞支架组(P<0.05)，细胞碱性磷酸酶活性高于单纯细胞组、细胞支架组。表明可注射型geneX人工骨复合骨髓间充质干细胞/转化生长因子β2具有良好组织相容性及促成骨分化能力。
배경：단일지가재료재불동정도불동범위내존재일사결점，인차근년래연구자선택복합지가재료，장량충혹다충구유일정호보특성적생물재료안일정방식여비례복합，구조출신형복합재료。 　　목적：탐토가주사형geneX인공골복합골수간충질간세포/전화생장인자β2적조직상용성급체외성골유도분화효과。 　　방법：장가주사형geneX인공골여토골수간충질간세포복합배양5 d，전경관찰인공골적조직상용성。취제3대성골유도배양적토골수간충질간세포분3조배양，단순세포조부가입성골유도분화배양기，세포지가조가입geneX인공골여성골유도분화배양기，연합조가입geneX인공골、전화생장인자β2여성골유도분화배양기，배양7，14，21 d진행세포형태학관찰、감성린산매활성검측、MTT검측、갑기백리향분람검측급천소홍염색관찰。 　　결과여결론：골수간충질간세포재가주사형geneX인공골표면첩벽생장，정성섬유세포형태，세포기질분비왕성。연합조세포증식、성골유도활성강우단순세포조、세포지가조(P<0.05)，세포감성린산매활성고우단순세포조、세포지가조。표명가주사형geneX인공골복합골수간충질간세포/전화생장인자β2구유량호조직상용성급촉성골분화능력。
BACKGROUND:Single scaffold materials have some shortcomings to some extent or in different ranges. Therefore, new composite materials are developed in recent year, which are compounded by two or more materials with complementary characteristics in a certain manner or ratio. OBJECTIVE:To investigate the histocompatibility and osteogenic induction of geneX artificial bone combined with bone marrow mesenchymal stem cel s and transforming growth factor beta 2 (TGF-β2). METHODS:Injectable geneX was co-cultured with bone marrow mesenchymal stem cel s for 5 days, and under the electron microscope, the histocompatibility of the artificial bone was observed. Passage 3 rabbit bone marrow mesenchymal stem cel s were divided into three groups:simple cel group cultured with osteogenic medium, cel scaffold group cultured with geneX+osteogenic medium, combined group cultured with geneX+TGF-β2+osteogenic medium. After 7, 14, 21 days, cel morphology, alkaline phosphatase activity detection, MTT detection, methyl thymol blue detection and alizarin red staining were performed. RESULTS AND CONCLUSION:After osteogenic induction, bone marrow mesenchymal stem cel s were fibroblast-like cel s and adhered to the surface of geneX bone with strong secretion of extracel ular matrix. Cel proliferation and osteogenic activity in the combined group were stronger than those in the simple cel and cel scaffold groups (P<0.05). The alkaline phosphatase activity was also higher in the combined group than the other two groups. These findings indicate that the geneX/bone marrow mesenchymal stem cel s/TGF-β2 composite has good histocompatibility and pro-osteogenic differentiation ability.