中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2015年
4期
234-237
,共4页
杨柳%姜南%钱洪军%徐扬阳
楊柳%薑南%錢洪軍%徐颺暘
양류%강남%전홍군%서양양
脂肪干细胞%膨体聚四氟乙烯%组织工程%血管生成
脂肪榦細胞%膨體聚四氟乙烯%組織工程%血管生成
지방간세포%팽체취사불을희%조직공정%혈관생성
Adipose derived stem cell%Expended polytetrafluoroethylenee%Tissue engineering%Angiogenesis
目的 评价人脂肪干细胞(hADSCs)复合膨体聚四氟乙烯(ePTFE)在SD大鼠体内的生长增殖情况,为临床运用hADSCs进行组织修复研究提供依据.方法 从健康成人脂肪抽吸术后的脂肪中获取hADSCs.按照完全随机化原则分为两组:A组为ePTFE材料接种hADSCs,B组仅为ePTFE材料,将两组移植于大鼠背部皮下,每只大鼠背上移植2个点(共10只).细胞-材料复合物在SD大鼠体内培养4周后,观察移植物外形、成活情况、湿重,应用苏木精-伊红(HE)染色和CD31荧光染色观察细胞在支架上的生长情况及毛细血管分布,并计算毛细血管密度.结果 原代培养的hADSCs呈梭形生长,细胞活性良好.移植术后4周hADSCs均能于ePTFE支架的边缘及空隙内生长.A组移植物湿重(511.62±32.82) mg、B组(363.56±34.74) mg,差异有统计学意义(P＜0.05).CD31荧光染色显微镜下,A组高倍镜视野微血管密度(29.50±2.63)个、B组(12.00±2.26)个,差异有统计学意义(P＜0.05).A组纤维结缔组织增生较少,毛细血管生成较多.结论 hADSCs与ePTFE支架具有较好的相容性,其增殖不受ePTFE支架的影响,hADSCs可促进局部血管生成,并可用于组织工程缺损修复的研究.
目的 評價人脂肪榦細胞(hADSCs)複閤膨體聚四氟乙烯(ePTFE)在SD大鼠體內的生長增殖情況,為臨床運用hADSCs進行組織脩複研究提供依據.方法 從健康成人脂肪抽吸術後的脂肪中穫取hADSCs.按照完全隨機化原則分為兩組:A組為ePTFE材料接種hADSCs,B組僅為ePTFE材料,將兩組移植于大鼠揹部皮下,每隻大鼠揹上移植2箇點(共10隻).細胞-材料複閤物在SD大鼠體內培養4週後,觀察移植物外形、成活情況、濕重,應用囌木精-伊紅(HE)染色和CD31熒光染色觀察細胞在支架上的生長情況及毛細血管分佈,併計算毛細血管密度.結果 原代培養的hADSCs呈梭形生長,細胞活性良好.移植術後4週hADSCs均能于ePTFE支架的邊緣及空隙內生長.A組移植物濕重(511.62±32.82) mg、B組(363.56±34.74) mg,差異有統計學意義(P＜0.05).CD31熒光染色顯微鏡下,A組高倍鏡視野微血管密度(29.50±2.63)箇、B組(12.00±2.26)箇,差異有統計學意義(P＜0.05).A組纖維結締組織增生較少,毛細血管生成較多.結論 hADSCs與ePTFE支架具有較好的相容性,其增殖不受ePTFE支架的影響,hADSCs可促進跼部血管生成,併可用于組織工程缺損脩複的研究.
목적 평개인지방간세포(hADSCs)복합팽체취사불을희(ePTFE)재SD대서체내적생장증식정황,위림상운용hADSCs진행조직수복연구제공의거.방법 종건강성인지방추흡술후적지방중획취hADSCs.안조완전수궤화원칙분위량조:A조위ePTFE재료접충hADSCs,B조부위ePTFE재료,장량조이식우대서배부피하,매지대서배상이식2개점(공10지).세포-재료복합물재SD대서체내배양4주후,관찰이식물외형、성활정황、습중,응용소목정-이홍(HE)염색화CD31형광염색관찰세포재지가상적생장정황급모세혈관분포,병계산모세혈관밀도.결과 원대배양적hADSCs정사형생장,세포활성량호.이식술후4주hADSCs균능우ePTFE지가적변연급공극내생장.A조이식물습중(511.62±32.82) mg、B조(363.56±34.74) mg,차이유통계학의의(P＜0.05).CD31형광염색현미경하,A조고배경시야미혈관밀도(29.50±2.63)개、B조(12.00±2.26)개,차이유통계학의의(P＜0.05).A조섬유결체조직증생교소,모세혈관생성교다.결론 hADSCs여ePTFE지가구유교호적상용성,기증식불수ePTFE지가적영향,hADSCs가촉진국부혈관생성,병가용우조직공정결손수복적연구.
Objective To observe the growth of human adipose stem cells (hADSCs) co-culture with expanded polytetrafluoroethylenee (ePTFE) scaffold in vivo.Methods hADSCs were isolated from healthy human liposuction fat.The hADSCs were seeded onto the ePTFE scaffold as Group A,while the ePTFE scaffold without hADSCs as Group B,and the two groups were randomly implanted under the back skin of 10 SD rats.The transplant compound in two groups was harvested at 4 weeks after implantation.Wet weight of transplanted compound was measured.After HE and immunofluorescence CD31 staining,blood vessel density and fibrous proliferation were observed and evaluated.Results Primary cultured hADSCs were spindle-shaped cells.The histological study found that after cultured in vivo,hADSCs could grow in the space of the ePTFE scaffold,several cells were found at the edge of the scaffolds and parts of cells had grown into the inside even the whole layer of the scaffolds.The wet weight of transplanted compound in group A (511.62±32.82) mg was significantly higher than that in group B (363.56±34.74) mg,(P＜0.05).Histological and immunofluorescence analysis showed the blood vessel density was (29.50±2.63)/HPL in group A,(12.00±2.26)/HPL in group B.Compared with group B,group A had significantly higher blood vessel density and lower fibrosis counts (P＜0.05).Conclusions hADSCs have a good biocompatibility with ePTFE scaffold.ePTFE scaffold has no negative effect on the proliferation of hADSCs.
