CAJ | 학술논문

背景：课题组前期研究已证实心肌干细胞移植中期(6周)能有效改善心肌梗死大鼠的心电生理稳定性和室颤阈值。 　　目的：比较心肌干细胞和骨髓间充质干细胞移植对心肌梗死大鼠心电生理学稳定性和室颤阈值的短期疗效差异。方法：取30只健康雄性SD大鼠，开胸结扎大鼠的左前降支冠状动脉建立心肌梗死动物实验模型，随机分成3组，分别为心肌干细胞移植组，骨髓间充质干细胞移植组，PBS对照组，每组10只。分别于建模成功2周后在梗死区心肌内局部注射0.1 mL PBS悬浮的5×106 PKH26标记的心肌干细胞、0.1 mL PBS悬浮的5×106 　　结果与结论：心肌干细胞移植组单极电图纠正的激动恢复时间离散度、电刺激所激发的恶性心律失常及梗死区、梗死边缘区、非梗死区的室颤阈值与PBS组、骨髓间充质干细胞移植组相比差异有显著性意义(P<0.05)；骨髓间充质干细胞移植组单极电图纠正的激动恢复时间离散度及非梗死区的室颤阈值与PBS组相比差异有显著性意义。病理结果提示在心肌干细胞组、骨髓间充质干细胞组梗死边缘区发现有心肌干细胞、骨髓间充质干细胞的存在。通过组织免疫荧光检测，PKH26标记的心肌干细胞表达较多的缝隙连接蛋白43，而PKH26标记的骨髓间充质干细胞很少表达缝隙连接蛋白43，PBS 组不表达缝隙连接蛋白43。上述结果提示心肌干细胞移植短期内心电生理学特性改善的效应较骨髓间充质干细胞优越，且改善效应与缝隙连接蛋白43相关。 　　PKH26标记的骨髓间充质干细胞及0.1 mL PBS。植入2周后，再次开胸检测大鼠心脏梗死区、梗死边缘区、非梗死区的心电生理特性和室颤阈值。实验结束后，分离心脏梗死边缘区进行病理切片及荧光显微镜检查PHK26标记的心肌干细胞和骨髓间充质干细胞缝隙连接蛋白43表达情况。揹景：課題組前期研究已證實心肌榦細胞移植中期(6週)能有效改善心肌梗死大鼠的心電生理穩定性和室顫閾值。 　　目的：比較心肌榦細胞和骨髓間充質榦細胞移植對心肌梗死大鼠心電生理學穩定性和室顫閾值的短期療效差異。方法：取30隻健康雄性SD大鼠，開胸結扎大鼠的左前降支冠狀動脈建立心肌梗死動物實驗模型，隨機分成3組，分彆為心肌榦細胞移植組，骨髓間充質榦細胞移植組，PBS對照組，每組10隻。分彆于建模成功2週後在梗死區心肌內跼部註射0.1 mL PBS懸浮的5×106 PKH26標記的心肌榦細胞、0.1 mL PBS懸浮的5×106 　　結果與結論：心肌榦細胞移植組單極電圖糾正的激動恢複時間離散度、電刺激所激髮的噁性心律失常及梗死區、梗死邊緣區、非梗死區的室顫閾值與PBS組、骨髓間充質榦細胞移植組相比差異有顯著性意義(P<0.05)；骨髓間充質榦細胞移植組單極電圖糾正的激動恢複時間離散度及非梗死區的室顫閾值與PBS組相比差異有顯著性意義。病理結果提示在心肌榦細胞組、骨髓間充質榦細胞組梗死邊緣區髮現有心肌榦細胞、骨髓間充質榦細胞的存在。通過組織免疫熒光檢測，PKH26標記的心肌榦細胞錶達較多的縫隙連接蛋白43，而PKH26標記的骨髓間充質榦細胞很少錶達縫隙連接蛋白43，PBS 組不錶達縫隙連接蛋白43。上述結果提示心肌榦細胞移植短期內心電生理學特性改善的效應較骨髓間充質榦細胞優越，且改善效應與縫隙連接蛋白43相關。 　　PKH26標記的骨髓間充質榦細胞及0.1 mL PBS。植入2週後，再次開胸檢測大鼠心髒梗死區、梗死邊緣區、非梗死區的心電生理特性和室顫閾值。實驗結束後，分離心髒梗死邊緣區進行病理切片及熒光顯微鏡檢查PHK26標記的心肌榦細胞和骨髓間充質榦細胞縫隙連接蛋白43錶達情況。
배경：과제조전기연구이증실심기간세포이식중기(6주)능유효개선심기경사대서적심전생리은정성화실전역치。 　　목적：비교심기간세포화골수간충질간세포이식대심기경사대서심전생이학은정성화실전역치적단기료효차이。방법：취30지건강웅성SD대서，개흉결찰대서적좌전강지관상동맥건립심기경사동물실험모형，수궤분성3조，분별위심기간세포이식조，골수간충질간세포이식조，PBS대조조，매조10지。분별우건모성공2주후재경사구심기내국부주사0.1 mL PBS현부적5×106 PKH26표기적심기간세포、0.1 mL PBS현부적5×106 　　결과여결론：심기간세포이식조단겁전도규정적격동회복시간리산도、전자격소격발적악성심률실상급경사구、경사변연구、비경사구적실전역치여PBS조、골수간충질간세포이식조상비차이유현저성의의(P<0.05)；골수간충질간세포이식조단겁전도규정적격동회복시간리산도급비경사구적실전역치여PBS조상비차이유현저성의의。병리결과제시재심기간세포조、골수간충질간세포조경사변연구발현유심기간세포、골수간충질간세포적존재。통과조직면역형광검측，PKH26표기적심기간세포표체교다적봉극련접단백43，이PKH26표기적골수간충질간세포흔소표체봉극련접단백43，PBS 조불표체봉극련접단백43。상술결과제시심기간세포이식단기내심전생이학특성개선적효응교골수간충질간세포우월，차개선효응여봉극련접단백43상관。 　　PKH26표기적골수간충질간세포급0.1 mL PBS。식입2주후，재차개흉검측대서심장경사구、경사변연구、비경사구적심전생리특성화실전역치。실험결속후，분리심장경사변연구진행병리절편급형광현미경검사PHK26표기적심기간세포화골수간충질간세포봉극련접단백43표체정황。
BACKGROUND:Our previous work demonstrated that cardiac stem cel s (CSCs) transplantation could significantly improve the electrophysiological stability and ventricular fibril ation threshold in rats with myocardial infarction. OBJECTIVE:To compare the influence of CSCs and bone marrow mesenchymal stem cel s (BMSCs) transplantation on the electrophysiological stability and ventricular fibril ation threshold in rats with myocardial infarction in the short term. METHODS:Thirty male healthy Sprague-Dawley rats were selected to make myocardial infarction models induced by the left anterior descending coronary artery ligation. Then, animals were randomly divided into three groups, CSCs group, BMSCs group and the PBS group, with 10 rats in each group. Two weeks after modeling, animals were respectively given the injection of 5×106 CSCs labeled with PKH26 in 0.1 mL PBS, 5×106 BMSCs labeled with PKH26 in 0.1 mL PBS or 0.1 mL PBS alone into the infracted anterior ventricular free wal . Two weeks after intervention, the electrophysiological characteristics and ventricular fibril ation threshold were measured respectively at the infarct zone, the infarct marginal zone and the non-infarct zone. Labeled CSCs and BMSCs were detected, and the expression of connexin-43 was examined in 5 μm cryostat sections from the infarct marginal zone of each heart. RESULTS AND CONCLUSION:Compared with the BMSCs group and PBS group, significant differences were revealed in the correct unipolar electrograms activation recovery time dispersion (ARTcd), electrical stimulation-induced malignant ventricular arrhythmias and ventricular fibril ation threshold at the infarct zone, the infarct marginal zone and the non-infarct zone in the CSCs group (P<0.05). Obvious differences were discovered in the ARTcd, electrical stimulation-induced malignant ventricular arrhythmias and ventricular fibril ation threshold on the non-infarct area in the BMSCs group in contrast to the PBS group. Labeled CSCs or BMSCs were identified at the infarct marginal zone and expressed connexin-43. Connexin-43 was abundantly expressed in the CSCs group whereas it was rarely expressed in the BMSCs group, and even not expressed in the PBS group. These findings suggest that CSCs are superior to BMSCs in modulating the electrophysiological stability and the ventricular fibril ation threshold in the short term after transplantation, which is closely correlated with the expression of connexin-43.