CAJ | 학술논문

背景：以往大多采用转化生长因子β1诱导骨髓间充质干细胞向软骨细胞分化，但诱导效果不佳。 　　目的：对比分析骨髓间充质干细胞与关节软骨细胞共培养诱导、转化生长因子β1诱导骨髓间充质干细胞分化为软骨样细胞的效果。 　　方法：获取SD大鼠关节软骨细胞和骨髓间充质干细胞，分别设置1∶2，1∶1，2∶1浓度组，以转化生长因子β1诱导组为对照。经诱导培养20 d后MTT法检测细胞活力，阿利新蓝比色法检测氨基聚糖含量，Western Blot检测Ⅱ型胶原蛋白表达。 　　结果与结论：转化生长因子β1组的吸光度值显著小于软骨细胞和骨髓间充质干细胞1∶1组和软骨细胞和骨髓间充质干细胞2∶1组(P<0.05)。转化生长因子β1组的氨基聚糖含量和Ⅱ型胶原蛋白表达显著低于软骨细胞和骨髓间充质干细胞(1∶2，1∶1，2∶1)组。软骨细胞和骨髓间充质干细胞1∶1组与软骨细胞和骨髓间充质干细胞2∶1组之间各指标比较差异无显著性意义(P >0.05)。结果表明关节软骨细胞与骨髓间充质干细胞共培养，可使骨髓间充质干细胞向软骨细胞分化，且骨髓间充质干细胞对软骨细胞诱导存在饱和现象。
배경：이왕대다채용전화생장인자β1유도골수간충질간세포향연골세포분화，단유도효과불가。 　　목적：대비분석골수간충질간세포여관절연골세포공배양유도、전화생장인자β1유도골수간충질간세포분화위연골양세포적효과。 　　방법：획취SD대서관절연골세포화골수간충질간세포，분별설치1∶2，1∶1，2∶1농도조，이전화생장인자β1유도조위대조。경유도배양20 d후MTT법검측세포활력，아리신람비색법검측안기취당함량，Western Blot검측Ⅱ형효원단백표체。 　　결과여결론：전화생장인자β1조적흡광도치현저소우연골세포화골수간충질간세포1∶1조화연골세포화골수간충질간세포2∶1조(P<0.05)。전화생장인자β1조적안기취당함량화Ⅱ형효원단백표체현저저우연골세포화골수간충질간세포(1∶2，1∶1，2∶1)조。연골세포화골수간충질간세포1∶1조여연골세포화골수간충질간세포2∶1조지간각지표비교차이무현저성의의(P >0.05)。결과표명관절연골세포여골수간충질간세포공배양，가사골수간충질간세포향연골세포분화，차골수간충질간세포대연골세포유도존재포화현상。
BACKGROUND:Transformation growth factor beta 1 is mostly used to induce the chondrogenic differentiation of bone marrow mesenchymal stem cel s, but there is a poor induction efficacy. OBJECTIVE:To explore the chondrogenic differentiation of bone marrow mesenchymal stem cel s co-cultured with articular chondrocytes or induced by transforming growth factor beta 1. METHODS:Articular chondrocytes and bone marrow mesenchymal stem cel s from SD rats were harvested and divided into 1:2, 2:1, 1:1 concentration groups. Cel s induced by transforming growth factor beta 1 acted as control group. After 20 days of induced culture, MTT was used to detect cel viability, alcian blue colorimetric assay was applied to measure glycosaminoglycan content, and western blot assay was employed to determine the expression of col agen type II. RESULTS AND CONCLUSION:The absorbance value in the control group was significantly lower than that in the 1:1 and 2:1 groups (P<0.05). Glycosaminoglycan content and protein expression of col agen type II were also lower in the control group than the 1:2, 1:1, 2:1 groups. But there was no difference between 1:1 and 2:1 groups (P>0.05). The results show that bone marrow mesenchymal stem cel s co-cultured with articular chondrocytes can be induced to differentiate into chondrocytes, and meanwhile, there is a saturation phenomenon during the chondrogenic differentiation of bone marrow mesenchymal stem cel s.