CAJ | 학술논문

背景：脂肪干细胞具有来源广泛、含量丰富、容易获取，培养条件简单且扩增能力强等优点，有可能成为继骨髓间充质干细胞之后最具前景的骨组织工程种子细胞。 　　目的：探讨兔脂肪干细胞与脱钙骨支架材料复合修复尺骨缺损的可行性。 　　方法：构建兔尺骨缺损动物模型，将单纯脱钙骨材料植入右侧缺损区，设为对照组，将成骨诱导后的兔脂肪干细胞-脱钙骨支架材料复合物植入左侧缺损区，设为实验组。植入后12周，获取骨缺损处组织标本进行CT扫描和组织学检测。 　　结果与结论：经 CT 扫描，实验组骨缺损断端与材料的接合部位已不能清晰分辨，断端外侧可观察到平行骨痂。对照组脱钙骨未完全降解，可观察到清晰的骨断端，未观察到连续骨痂，骨缺损处大多为纤维连接，未出现骨性修复。经组织学检测，实验组缺损处为典型再生骨组织，存在骨细胞和骨陷窝以及骨小梁结构，骨细胞和骨陷窝数量较多，仅有部分骨小梁结构形成，并有少量胶原样结构穿插于再生骨组织之间；对照组有脱钙骨基质残留现象，并存在部分胶原纤维样组织，在边缘可观察到一定的骨膜成骨反应，但程度较轻，且未出现大片再生骨样组织。以上结果表明兔脂肪干细胞成骨诱导后与脱钙骨支架材料复合可以用于修复尺骨缺损。
배경：지방간세포구유래원엄범、함량봉부、용역획취，배양조건간단차확증능력강등우점，유가능성위계골수간충질간세포지후최구전경적골조직공정충자세포。 　　목적：탐토토지방간세포여탈개골지가재료복합수복척골결손적가행성。 　　방법：구건토척골결손동물모형，장단순탈개골재료식입우측결손구，설위대조조，장성골유도후적토지방간세포-탈개골지가재료복합물식입좌측결손구，설위실험조。식입후12주，획취골결손처조직표본진행CT소묘화조직학검측。 　　결과여결론：경 CT 소묘，실험조골결손단단여재료적접합부위이불능청석분변，단단외측가관찰도평행골가。대조조탈개골미완전강해，가관찰도청석적골단단，미관찰도련속골가，골결손처대다위섬유련접，미출현골성수복。경조직학검측，실험조결손처위전형재생골조직，존재골세포화골함와이급골소량결구，골세포화골함와수량교다，부유부분골소량결구형성，병유소량효원양결구천삽우재생골조직지간；대조조유탈개골기질잔류현상，병존재부분효원섬유양조직，재변연가관찰도일정적골막성골반응，단정도교경，차미출현대편재생골양조직。이상결과표명토지방간세포성골유도후여탈개골지가재료복합가이용우수복척골결손。
BACKGROUND:Adipose-derived stem cel s have a wide variety of sources and strong proliferation ability, which are easy to access and simple to culture. Therefore, adipose-derived stem cel s that are secondary to bone marrow mesenchymal stem cel s are expected to become the most promising seed cel s for bone tissue engineering. OBJECTIVE:To explore the feasibility of rabbit adipose-derived stem cel s with demineralized bone matrix to repair ulna defects. METHODS:Ulna defect model was made in rabbits. Demineralized bone matrix was implanted into the right defect region as control group. After osteogenic induction, rabbit adipose-derived stem cel s/demineralized bone matrix composite was implanted into the left defect region as experimental group. At 12 weeks after implantation, defect tissues were taken for CT scanning and histological detection. RESULTS AND CONCLUSION:CT results showed that there was unclear boundary between the broken ends of fractured bone and the composite material in the experimental group, and paral el cal uses out of the broken end could be seen. In the control group, the broken end was clearly seen and no cal us occurred continuously. Fibers were connected at the defect site, and no new bone occurred. Histological findings showed that typical regenerated bone tissues were seen in the experimental group with osteocytes, bone lacunae and bone trabeculae;there were more osteocytes and bone lacunae, but bone trabecula was only seen in a part of bone defects;a few of col agens interlarded the regenerated bone tissues. In the control group, the residual of demineralized bone matrix was seen as wel as some col agenous fibers, and periosteal bone formed a little, but no large amount of regenerated osteoid tissues were found. These findings indicate that under osteogenic induction, rabbit adipose-derived stem cel s combined with demineralized bone matrix are feasible to repair ulna defects.