CAJ | 학술논문

背景：随着基因工程以及肿瘤生物分子学等新兴学科的发展壮大，基因治疗肿瘤成为一种新的治疗模式。 　　目的：探讨KDR基因沉默对乳腺癌MCF-7细胞增殖能力和侵袭能力的影响。 　　方法：设计针对 KDR 基因的小分子干扰 RNA(siRNA)序列，转染人乳腺癌 MCF-7细胞，应用 RT-PCR 及Western blot法检测沉默KDR基因后MCF-7细胞KDR mRNA及蛋白的表达；应用流式细胞仪、CCK-8增殖实验、小室侵袭实验检测沉默KDR基因后乳腺癌MCF-7细胞的细胞周期、增殖能力、侵袭能力的变化。 　　结果与结论：KDR基因沉默48 h后，乳腺癌MCF-7细胞KDR mRNA及蛋白表达明显降低；乳腺癌MCF-7细胞停滞在G 0/G 1周期，S期的细胞数目减低，细胞增殖得到显著抑制，穿过滤膜的细胞数量明显减少。上述结果表明沉默KDR基因后乳腺癌MCF-7细胞的增殖、侵袭能力得到明显抑制，说明KDR基因沉默可能成为新的有效治疗乳腺癌的靶点。
배경：수착기인공정이급종류생물분자학등신흥학과적발전장대，기인치료종류성위일충신적치료모식。 　　목적：탐토KDR기인침묵대유선암MCF-7세포증식능력화침습능력적영향。 　　방법：설계침대 KDR 기인적소분자간우 RNA(siRNA)서렬，전염인유선암 MCF-7세포，응용 RT-PCR 급Western blot법검측침묵KDR기인후MCF-7세포KDR mRNA급단백적표체；응용류식세포의、CCK-8증식실험、소실침습실험검측침묵KDR기인후유선암MCF-7세포적세포주기、증식능력、침습능력적변화。 　　결과여결론：KDR기인침묵48 h후，유선암MCF-7세포KDR mRNA급단백표체명현강저；유선암MCF-7세포정체재G 0/G 1주기，S기적세포수목감저，세포증식득도현저억제，천과려막적세포수량명현감소。상술결과표명침묵KDR기인후유선암MCF-7세포적증식、침습능력득도명현억제，설명KDR기인침묵가능성위신적유효치료유선암적파점。
BACKGROUND:With the development of genetic engineering and tumor molecular biology, gene therapy for tumors has become a new treatment modality. OBJECTIVE:To explore the effect of the KDR gene silencing on the proliferation and invasion capacity of breast cancer MCF-7 cel s. METHODS:Interfering RNA (siRNA) sequences for smal molecule KDR gene was designed and transferred into human breast cancer MCF-7 cel s. Then, RT-PCR and western blot assay were used to detect the KDR mRNA and protein expression. Flow cytometry, cel counting kit-8 test and Transwel test were employed to detect the cel cycle, proliferative capacity and invasion capacity of breast cancer MCF-7 cel s after the KDR gene silencing. RESULTS AND CONCLUSION:After 48 hours of KDR silencing, the mRNA and protein expressions of KDR in MCF-7 cel s were decreased obviously;MCF-7 cel s arrested at G0/G1 stage and the number of cel s at S stage was reduced. Cel proliferation was inhibited significantly. The amount of cel s passing through the filtering membrane became less. After KDR gene silencing, the proliferation and invasion of breast cancer MCF-7 cancer stem cel s were inhibited remarkably, indicating that KDR gene silencing may be a new target for the effective treatment of breast cancer.