CAJ | 학술논문

背景：异种生物衍生骨保存了原骨组织的天然网状孔隙结构，且具有免疫原性低、细胞相容性好的特点。目的：验证异种生物衍生骨材料与骨髓间充质干细胞的生物相容性。 　　方法：取新鲜猪股骨制备生物衍生骨，扫描电镜观察材料结构。将第3代兔骨髓间充质干细胞以2×109 L-1的浓度接种于生物衍生骨材料的松质骨面，复合培养7d内，采用扫描电镜观察细胞生长情况；复合培养8d内，计数材料上附着的细胞数。 　　结果与结论：生物衍生骨表面粗糙，孔隙不规则并相互通联，构成网状结构。复合培养3 d，细胞在衍生骨材料表面发生附着，且细胞形态不均一；复合培养培养5 d，细胞连接成片呈层状生长，细胞之间紧密接触；复合培养7 d，细胞呈现多层生长状态，成堆生长，部分区域存在细胞外基质分泌现象。复合培养后，前2 d 为潜伏适应期，3-6d细胞生长呈出线性曲线，处于细胞生长对数期；第6天后，细胞生长曲线逐渐变得平缓，细胞增殖速度下降，增殖进入平台期。表明异种生物衍生骨材料与骨髓间充质干细胞具有良好的生物相容性。
배경：이충생물연생골보존료원골조직적천연망상공극결구，차구유면역원성저、세포상용성호적특점。목적：험증이충생물연생골재료여골수간충질간세포적생물상용성。 　　방법：취신선저고골제비생물연생골，소묘전경관찰재료결구。장제3대토골수간충질간세포이2×109 L-1적농도접충우생물연생골재료적송질골면，복합배양7d내，채용소묘전경관찰세포생장정황；복합배양8d내，계수재료상부착적세포수。 　　결과여결론：생물연생골표면조조，공극불규칙병상호통련，구성망상결구。복합배양3 d，세포재연생골재료표면발생부착，차세포형태불균일；복합배양배양5 d，세포련접성편정층상생장，세포지간긴밀접촉；복합배양7 d，세포정현다층생장상태，성퇴생장，부분구역존재세포외기질분비현상。복합배양후，전2 d 위잠복괄응기，3-6d세포생장정출선성곡선，처우세포생장대수기；제6천후，세포생장곡선축점변득평완，세포증식속도하강，증식진입평태기。표명이충생물연생골재료여골수간충질간세포구유량호적생물상용성。
BACKGROUND:Bio-derived xenograft bone has natural pore structure of the bone, low immunogenicity, and good cytocompatibility. OBJECTIVE:To verify the biocompatibility of bio-derived xenograft bone with bone marrow mesenchymal stem cel s. METHODS:Fresh pig femoral bone was col ected to prepare bio-derived bone. Scanning electron microscope was used to observe the material structure. Passage 3 rabbit bone marrow mesenchymal stem cel s at a density of 2×109/L were inoculated into the cancel ous bone surface of the bio-dervied bone and cultured for 7 days. Cel growth was detected using scanning electron microscope. After culture for 8 days, cel number was counted. RESULTS AND CONCLUSION:The bio-derived bone had rough surface and irregularly interconnected pores constructing a mesh-like structure. After 3 days of compound culture, cel s had irregular shapes and adhered to the surface of bio-derived bone;after 5 days of culture, cel s were closely interconnected to form a layered growth;after 7 days of culture, cel s exhibited multilayered growth and the extracel ular matrix secreted local y. Under compound culture, the former 2 days were latent period, 3-6 days were logarithmic phase, and from the 6 th day, the cel growth curve became smooth gradual y and the cel proliferation decreased and entered into the plateau period. These findings indicate that the bio-derived xenograft bone has good biocompatibility with bone marrow mesenchymal stem cel s.