CAJ | 학술논문

背景：已有研究显示在大鼠肾移植时连续给予1×107细胞浓度的骨髓间充质干细胞可诱导免疫耐受，但临床上尚未发现姜黄素对肾移植具有免疫调节作用。 　　目的：在对肾移植后的大鼠给予骨髓间充质干细胞的基础上，同时给予不同剂量姜黄素，观察其免疫耐受的效果。 　　方法：建立大鼠肾移植模型，随机分为4组：纯肾移植组，无治疗措施；骨髓间充质干细胞组：关腹前左骼静脉注射1×107/kg骨髓间充质干细胞，第2天起尾静脉注射等量细胞，共10 d；骨髓间充质干细胞+低/高剂 　　结果与结论：大鼠经肾移植后，纯肾移植组中肾小管上皮细胞以及间质细胞的转化生长因子β1蛋白表达、白细胞介素2，6水平均高于其他各组(P <0.05)。与骨髓间充质干细胞组比较，骨髓间充质干细胞+低/高剂量姜黄素组的转化生长因子β1的蛋白表达降低(P<0.05)，血清白细胞介素2，6水平均低于骨髓间充质干细胞组(P<0.05)。结果证实，大鼠肾移植时同时给予姜黄素与骨髓间充质干细胞注射，可有效地进行免疫调节，抑制性免疫排斥反应的发生，保护肾功能。 　　量姜黄素组：除给予骨髓间充质干细胞外，分别以2，10 mg/kg姜黄素溶液灌胃，共10 d。免疫组织化学染色法检测各组肾组织中转化生长因子β1的蛋白表达水平，酶联免疫吸附法检测血清中白细胞介素2，6水平。
배경：이유연구현시재대서신이식시련속급여1×107세포농도적골수간충질간세포가유도면역내수，단림상상상미발현강황소대신이식구유면역조절작용。 　　목적：재대신이식후적대서급여골수간충질간세포적기출상，동시급여불동제량강황소，관찰기면역내수적효과。 　　방법：건립대서신이식모형，수궤분위4조：순신이식조，무치료조시；골수간충질간세포조：관복전좌격정맥주사1×107/kg골수간충질간세포，제2천기미정맥주사등량세포，공10 d；골수간충질간세포+저/고제 　　결과여결론：대서경신이식후，순신이식조중신소관상피세포이급간질세포적전화생장인자β1단백표체、백세포개소2，6수평균고우기타각조(P <0.05)。여골수간충질간세포조비교，골수간충질간세포+저/고제량강황소조적전화생장인자β1적단백표체강저(P<0.05)，혈청백세포개소2，6수평균저우골수간충질간세포조(P<0.05)。결과증실，대서신이식시동시급여강황소여골수간충질간세포주사，가유효지진행면역조절，억제성면역배척반응적발생，보호신공능。 　　량강황소조：제급여골수간충질간세포외，분별이2，10 mg/kg강황소용액관위，공10 d。면역조직화학염색법검측각조신조직중전화생장인자β1적단백표체수평，매련면역흡부법검측혈청중백세포개소2，6수평。
BACKGROUND:Previous studies have shown that continuous administration of 1×107 of bone marrow mesenchymal stem cel s can induce immune tolerance in rats undergoing kidney transplantation, but it is not yet found clinical y that curcumin exerts an on immunomodulatory effect on kidney transplantation. OBJECTIVE:To investigate the effects of different concentrations of curcumin on bone marrow mesenchymal stem cel s-induced immune tolerance in rats after kidney transplantation. METHODS:Rat model of kidney transplantation was made, and rat models were randomly divided into four groups:transplantation group with no treatment;bone marrow mesenchymal stem cel s group (cel group) injected with 1×107/kg bone marrow mesenchymal stem cel s via the left iliac vein (before peritoneal suture) and tail vein (from the 2nd day) for 10 days;bone marrow mesenchymal stem cel s+low/high dosage of curcumin groups (low/high dosage curcumin groups) injected intragastrical y with 2 or 10 mg/kg curcumin combined with injection of bone marrow mesenchymal stem cel s for 10 days. Transforming growth factor-β1 protein expression in the kidney tissues was examined by immunohistochemical staining. The concentrations of interleukin-2 and interleukin-6 in serum were detected by ELISA assay. RESULTS AND CONCLUSION:After kidney transplantation, the protein expression of transforming growth factor-β1 in renal tubular epithelial cel s and renal interstitial cel s as wel as the concentrations of interleukin-2and interleukin-6 in serum were significantly higher in the transplantation group than the other groups (P<0.05). Compared with the cel transplantation group, the protein expression of transforming growth factor-β1 as wel as the concentrations of interleukin-2 and interleukin-6 reduced significantly in the low/high dosage curcumin groups (P<0.05). These findings indicate that simultaneous administration of curcumin and bone marrow mesenchymal stem cel s can effectively inhibit immune rejection reaction and improve renal function in rats after kidney transplantation.