CAJ | 학술논문

背景：临床应用补肾活血中药龙鳖胶囊治疗骨关节炎取得满意的疗效，但其作用机制尚不明确。目的：观察龙鳖胶囊对大鼠骨髓间充质干细胞增殖活性的影响。 　　方法：采用全骨髓贴壁培养方法分离培养 SD 大鼠骨髓间充质干细胞，倒置显微镜进行形态学观察，流式细胞术检测细胞表面标志表达。用含龙鳖胶囊的完全培养基培养第4代骨髓间充质干细胞，药物质量浓度分为5，1 g/L和250，50，10 mg/L，作用24 h后用 MTT 法测定细胞活力。 　　结果与结论：原代细胞呈现不规则形状贴壁生长，第2代细胞呈典型的纤维状，第3代细胞呈长纤维状、漩涡式生长。第4代骨髓间充质干细胞的表面分子CD29、CD90呈强阳性表达，CD44呈阳性表达，CD45、CD34呈阴性表达。5 g/L 和1 g/L质量浓度的龙鳖胶囊溶液可促进骨髓间充质干细胞增殖。结果表明龙鳖胶囊可能是通过促进骨髓间充质干细胞的增殖和分化，进而发挥治疗骨关节炎的作用。
배경：림상응용보신활혈중약룡별효낭치료골관절염취득만의적료효，단기작용궤제상불명학。목적：관찰룡별효낭대대서골수간충질간세포증식활성적영향。 　　방법：채용전골수첩벽배양방법분리배양 SD 대서골수간충질간세포，도치현미경진행형태학관찰，류식세포술검측세포표면표지표체。용함룡별효낭적완전배양기배양제4대골수간충질간세포，약물질량농도분위5，1 g/L화250，50，10 mg/L，작용24 h후용 MTT 법측정세포활력。 　　결과여결론：원대세포정현불규칙형상첩벽생장，제2대세포정전형적섬유상，제3대세포정장섬유상、선와식생장。제4대골수간충질간세포적표면분자CD29、CD90정강양성표체，CD44정양성표체，CD45、CD34정음성표체。5 g/L 화1 g/L질량농도적룡별효낭용액가촉진골수간충질간세포증식。결과표명룡별효낭가능시통과촉진골수간충질간세포적증식화분화，진이발휘치료골관절염적작용。
BACKGROUND:Longbie Capsule has satisfactory outcomes in the treatment of osteoarthritis, but its mechanism is stil unclear. OBJECTIVE:To study the effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cel s. METHODS:The bone marrow mesenchymal stem cel s from SD rats were separated and expanded by whole adherence culture, then subcultured and confirmed by morphological observation and flow cytometry. Passage 4 cel s were cultured in complete media containing 5 g/L, 1 g/L, 250 mg/L, 50 mg/L, 10 mg/L Longbie Capsule, respectively, for 24 hours. Then, MTT assay was used to detect cel viability. RESULTS AND CONCLUSION:The primary cel s were adherent cel s characterized by irregular shape, passage 2 cel s were typical y fibrous-shaped, passage 3 cel s grew in long fibrous and swirl-type shape. Passage 4 cel s were strongly positive for CD29 and CD90, positive for CD44, and negative for CD34 and CD45. 5 g/L and 1 g/L Longbie Capsule promoted the proliferation of bone marrow mesenchymal stem cel s. These findings indicate that Longbie Capsule may promote the proliferation and differentiation of bone marrow mesenchymal stem cel s, thereby playing a therapeutic effect on osteoarthritis.