CAJ | 학술논문

为了提高辅酶Q10的产生菌米曲霉菌的基因组改组育种效率，以提高辅酶Q10的产量，本试验对米曲霉原生质体的制备条件进行了研究。通过对影响制备效果的条件，即米曲霉的菌丝菌龄、酶解温度、时间和原生质体稳定剂的浓度四个因素进行全面优化，并利用正交试验确定了原生质体制备的适宜条件：菌龄为12 h、原生质体渗透压稳定剂NaCl浓度为0．85 mol／L、酶解时间为3．5 min、酶解温度为30℃时，米曲霉原生质体制备效果最好，所得原生质体为6．3×106个／mL，再生率达到38．06％。较高的原生质体生成量和再生率为该菌后序的基因组改组研究奠定了基础。
위료제고보매Q10적산생균미곡매균적기인조개조육충효솔，이제고보매Q10적산량，본시험대미곡매원생질체적제비조건진행료연구。통과대영향제비효과적조건，즉미곡매적균사균령、매해온도、시간화원생질체은정제적농도사개인소진행전면우화，병이용정교시험학정료원생질체제비적괄의조건：균령위12 h、원생질체삼투압은정제NaCl농도위0．85 mol／L、매해시간위3．5 min、매해온도위30℃시，미곡매원생질체제비효과최호，소득원생질체위6．3×106개／mL，재생솔체도38．06％。교고적원생질체생성량화재생솔위해균후서적기인조개조연구전정료기출。
In order to improve the breeding efficiency of As pergillus oryzae by genome shuffling methods to produce more coenzyme Q10 products,the processing conditions to generate As pergillus oryzae protoplasts are studied by optimum seeking method and orthogonal experiment in this paper. The results show that the optimal conditions for the protoplast formation and regeneration are as follows:the age of the mycelium of As pergillus oryzae for protoplasts is 12 h,the hydrolysis reaction temperature is 30 ℃,the hydrolysis reaction time is 3 .5 min,the protoplast osmotic stabilizer NaCl concentration is 0.85 mol/L,and under the optimal conditions,the protoplast formation amount and regeneration rate could respectively approach 6.3 × 106 cells/mL and 38.06%.The high yield and high regeneration rate lay a good foundation for the genetic improvement of industrial coenzyme Q10-producing strain.