中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2015年
8期
43-47
,共5页
刘芸%任慧婧%黄锴%王珂佳%胡美忠
劉蕓%任慧婧%黃鍇%王珂佳%鬍美忠
류예%임혜청%황개%왕가가%호미충
芫荽%总黄酮%大孔树脂%抗氧化%抗菌
芫荽%總黃酮%大孔樹脂%抗氧化%抗菌
원유%총황동%대공수지%항양화%항균
Coriandrumsativum L.%total flavonoids%macroporous resin%antioxidant%antimicrobial
目的：研究芫荽总黄酮的大孔树脂纯化工艺及总黄酮抗氧化、抗菌活性。方法：通过筛选优化大孔树脂型号，优化大孔树脂提取芫荽总黄酮条件，利用DPP H 芫荽总黄酮抗氧化活性，琼脂扩散法测试芫荽总黄酮抗菌活性。结果：AB-8树脂对芫荽总黄酮有较好的吸附和解吸效果，最优工艺条件为：芫荽总黄酮上样液的质量浓度为1．5 mg／mL，上样速率为2 mL／min，洗脱剂为70％乙醇，洗脱剂用量为4 BV，洗脱速率为2 mL／min，芫荽黄酮提取物清除DPPH ·自由基的EC50为1．5 mg／mL。对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌、绿脓杆菌、黑曲霉均有抗菌活性，但是对酿酒酵母无效。结论：AB-8型大孔树脂富集芫荽总黄酮效果最佳。
目的：研究芫荽總黃酮的大孔樹脂純化工藝及總黃酮抗氧化、抗菌活性。方法：通過篩選優化大孔樹脂型號，優化大孔樹脂提取芫荽總黃酮條件，利用DPP H 芫荽總黃酮抗氧化活性，瓊脂擴散法測試芫荽總黃酮抗菌活性。結果：AB-8樹脂對芫荽總黃酮有較好的吸附和解吸效果，最優工藝條件為：芫荽總黃酮上樣液的質量濃度為1．5 mg／mL，上樣速率為2 mL／min，洗脫劑為70％乙醇，洗脫劑用量為4 BV，洗脫速率為2 mL／min，芫荽黃酮提取物清除DPPH ·自由基的EC50為1．5 mg／mL。對枯草芽孢桿菌、金黃色葡萄毬菌、大腸桿菌、綠膿桿菌、黑麯黴均有抗菌活性，但是對釀酒酵母無效。結論：AB-8型大孔樹脂富集芫荽總黃酮效果最佳。
목적：연구원유총황동적대공수지순화공예급총황동항양화、항균활성。방법：통과사선우화대공수지형호，우화대공수지제취원유총황동조건，이용DPP H 원유총황동항양화활성，경지확산법측시원유총황동항균활성。결과：AB-8수지대원유총황동유교호적흡부화해흡효과，최우공예조건위：원유총황동상양액적질량농도위1．5 mg／mL，상양속솔위2 mL／min，세탈제위70％을순，세탈제용량위4 BV，세탈속솔위2 mL／min，원유황동제취물청제DPPH ·자유기적EC50위1．5 mg／mL。대고초아포간균、금황색포도구균、대장간균、록농간균、흑곡매균유항균활성，단시대양주효모무효。결론：AB-8형대공수지부집원유총황동효과최가。
Obj ective:To investigate the purification technology of total flavonoids with macroporous resin and to study the antibacterial and antioxidant activity of total flavonoids from Coriandrum sativum L..Method:Screen the optimal macroporous resin and optimize the purification technology of total flavonoids from Coriandrumsativum L.,then use the DPPH method to test the antioxidant activity and agar-diffusion method to test the antibacterial activity of total flavonoids from Coriandrum sativum L..Results:AB-8 resin has excellent effect on the adsorption and desorption of total flavonoids from Coriandrumsativum L..The sample concentration is 1 .5 mg/mL and the rate is 2 mL/min,the eluent is 70% ethanol of 4 BV and rate of 2 mL/min,which are the optimal purification process conditions.The total flavonoids from Coriandrumsativum L.also has excellent antioxidant activity (EC50 = 1 .5 mg/mL ),and the antibacterial activity against Bacillussubtilis, Staphylococcusaureus,Escherichiacoli,Pesudomonasaeruginosa,Aspergillusniger, but not against Saccharomycescerevisiae.
