CAJ | 학술논문

基于生物量在线检测技术对谷氨酸发酵工艺进行研究，经Plackett-Burman 试验设计发现硫酸镁、维生素B1和玉米浆为三个影响谷氨酸产酸的主要因素。采用最陡爬坡实验逼近最大响应区，借助Box-Behnken试验设计进行响应面优化回归分析，探讨该工艺的最佳条件值，结果显示：谷氨酸产酸最大理论值为86．52 g／L，硫酸镁最佳浓度4．26 g／L，维生素B1最佳浓度18．03 mg／L，玉米浆最佳浓度33．91 mL／L，在10 L发酵罐S9114谷氨酸棒状杆菌32℃发酵实验上验证，实测值与理论值呈现很好的一致性，在优化条件下谷氨酸产酸提高了19．7％，为谷氨酸发酵提供了参考。
기우생물량재선검측기술대곡안산발효공예진행연구，경Plackett-Burman 시험설계발현류산미、유생소B1화옥미장위삼개영향곡안산산산적주요인소。채용최두파파실험핍근최대향응구，차조Box-Behnken시험설계진행향응면우화회귀분석，탐토해공예적최가조건치，결과현시：곡안산산산최대이론치위86．52 g／L，류산미최가농도4．26 g／L，유생소B1최가농도18．03 mg／L，옥미장최가농도33．91 mL／L，재10 L발효관S9114곡안산봉상간균32℃발효실험상험증，실측치여이론치정현흔호적일치성，재우화조건하곡안산산산제고료19．7％，위곡안산발효제공료삼고。
Based on biomass on-line detection technology of glutamic acid fermentation,a process is studied through Plackett-Burman test design,it is found that magnesium sulfate,vitamin B1 and corn starch as the three main factors affect glutamic acid production.Approaching the maximum response of the steepest uphill experiment area,a Box-Behnken experimental design is applied to analyze the response surface optimization,investigate the best conditions of the process value and the results show that the maximum theoretical value of glutamic acid yield is 86.52 g/L,the optimal concentration of magnesium sulfate,vitamin B1 and corn slurry is 4.26 g/L,18.03 mg/L,33.91 mL/L respectively, which is verified by the fermentation experiment in 10 L fermentation tank with S9114 glutamate corynebacterium at 32 ℃,the measured values and the theoretical values are close to.Under the condition,the glutamic acid yield is increased by 1 9 .7%, provides reference for glutamic acid fermentation.