蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
8期
1108-1110
,共3页
丙型肝炎%丙型肝炎抗体%基因型
丙型肝炎%丙型肝炎抗體%基因型
병형간염%병형간염항체%기인형
hepatitis C%hepatitis C antibody%genotype
目的：：对5080例丙型肝炎患者Anti-HCV、HCV-RNA及基因分型三项检验结果进行分析。方法：采用增强发光免疫法检测Anti-HCV；实时荧光定量PCR法检验HCV-RNA；HCV-RNA含量≥103的标本同时进行基因分型检测。结果：5080例丙型肝炎患者中Anti-HCV检测阳性率为99.8％,HCV-RNA阳性率为49.1％。 HCV-RNA阳性患者年龄和抗体含量均明显高于HCV-RNA阴性患者(P<0.01)。在进行基因分型的1723例HCV-RNA阳性标本中,1b、2a分别占59.49％和36.20％,两型总占比达到95.69％；另外3b和4不常见型以及1b/2a、1b/4混合感染也检测到。结论：Anti-HCV含量与患者的病毒载量之间缺乏相关性,不能作为丙型肝炎治疗过程中的动态观察指标；HCV-RNA定量适于丙型肝炎疗效评价和预后判断指标；临床丙型肝炎患者中主要基因型是1b、2a型,对HCV-RNA阳性患者进行基因分型,可为丙型肝炎的个性化治疗提供决策依据。
目的：：對5080例丙型肝炎患者Anti-HCV、HCV-RNA及基因分型三項檢驗結果進行分析。方法：採用增彊髮光免疫法檢測Anti-HCV；實時熒光定量PCR法檢驗HCV-RNA；HCV-RNA含量≥103的標本同時進行基因分型檢測。結果：5080例丙型肝炎患者中Anti-HCV檢測暘性率為99.8％,HCV-RNA暘性率為49.1％。 HCV-RNA暘性患者年齡和抗體含量均明顯高于HCV-RNA陰性患者(P<0.01)。在進行基因分型的1723例HCV-RNA暘性標本中,1b、2a分彆佔59.49％和36.20％,兩型總佔比達到95.69％；另外3b和4不常見型以及1b/2a、1b/4混閤感染也檢測到。結論：Anti-HCV含量與患者的病毒載量之間缺乏相關性,不能作為丙型肝炎治療過程中的動態觀察指標；HCV-RNA定量適于丙型肝炎療效評價和預後判斷指標；臨床丙型肝炎患者中主要基因型是1b、2a型,對HCV-RNA暘性患者進行基因分型,可為丙型肝炎的箇性化治療提供決策依據。
목적：：대5080례병형간염환자Anti-HCV、HCV-RNA급기인분형삼항검험결과진행분석。방법：채용증강발광면역법검측Anti-HCV；실시형광정량PCR법검험HCV-RNA；HCV-RNA함량≥103적표본동시진행기인분형검측。결과：5080례병형간염환자중Anti-HCV검측양성솔위99.8％,HCV-RNA양성솔위49.1％。 HCV-RNA양성환자년령화항체함량균명현고우HCV-RNA음성환자(P<0.01)。재진행기인분형적1723례HCV-RNA양성표본중,1b、2a분별점59.49％화36.20％,량형총점비체도95.69％；령외3b화4불상견형이급1b/2a、1b/4혼합감염야검측도。결론：Anti-HCV함량여환자적병독재량지간결핍상관성,불능작위병형간염치료과정중적동태관찰지표；HCV-RNA정량괄우병형간염료효평개화예후판단지표；림상병형간염환자중주요기인형시1b、2a형,대HCV-RNA양성환자진행기인분형,가위병형간염적개성화치료제공결책의거。
Objective:To analyze the hepatitis C antibody( Anti-HCV) ,HCV-RNA and genotype in 5 080 patients with hepatitis C. Methods:The levels of Anti-HCV and HCV-RNA in all patients were determined by automatic enhanced luminescence immunity analyzer and quantitative fluorescent PCR,respectively. The genotypes in the patients with more than or equal to 103 of HCV-RNA were detected. Results:The positive rates of Anti-HCV and HCV-RNA were 99. 8% and 49. 1%,respectively. The ages and antibody content in positive HCV-RNA patients were significantly higher than those in the negative HCV-RNA patients(P<0. 01). Among the 1 723 positive HCV-RNA specimens,the 1b and 2a genotypes accounted for 59. 49% and 36. 20%,respectively,the total ratio of 1b and 2a genotypes was 95. 69%. The unusual types of 3b and 4,and mixed infection types of 1b/2a and 1b/4 also were detected. Conclusions:The Anti-HCV content and viral load are lack of correlation, which can not be used as the observation indexes in estimating the treatment. The quantitation of HCV-RNA can be used to evaluate the curative effect and prognosis of patients with hepatitis C. The 1b and 2a are the major genotypes in patients with hepatitis C. To analyze the genotype of the positive HCV-RNA patients can provide the basis in personalized therapy.
