蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
8期
1093-1096
,共4页
贺平%张利%张奎虎%朱美容
賀平%張利%張奎虎%硃美容
하평%장리%장규호%주미용
维生素A%反相超高效液相色谱%内标法
維生素A%反相超高效液相色譜%內標法
유생소A%반상초고효액상색보%내표법
vitamin A%reversed-phase ultra performance liquid chromatography%internal standard
目的：：建立反相超高效液相色谱(UPLC)内标法测定儿童血清中维生素A(VitA)水平的方法,以了解学龄儿童VitA营养状况,为制定膳食营养计划提供参考。方法：阜阳市颍上县学龄儿童100μl血清加入100μl内标后,振荡混匀,正己烷1.0 ml萃取后离心,取上清液用N2吹干,200μl无水乙醇复溶,UPLC法测定。色谱条件为色谱柱：Waters C18柱(2.1 mm ×100 mm,1.7μm)；二级阵列管检测器；流动相：乙腈：水(94：6)临用前脱气；检测波长325 nm；柱温：40℃；流速：0.3 ml/min；进样2μl。结果：360例学龄儿童血清VitA含量在0.0728~0.728μg/ml范围内呈良好的线性关系(r>0.999),VitA的平均值为39.60μg/dl,CV<5％,VitA的最低检测限为0.020μg/ml,测定在4.0 min内完成。360例学龄儿童中VitA含量≤20μg/dl占总人数的1.11％,VitA水平20~30μg/dl占总人数的19.17％,VitA水平≥30μg/dl占总人数的79.72％。结论：调查结果总体满足膳食营养计划的要求。反相UPLC内标法检测儿童血清中VitA的水平,方法快速、灵敏、准确,重复性好,样品处理简单宜行。
目的：：建立反相超高效液相色譜(UPLC)內標法測定兒童血清中維生素A(VitA)水平的方法,以瞭解學齡兒童VitA營養狀況,為製定膳食營養計劃提供參攷。方法：阜暘市潁上縣學齡兒童100μl血清加入100μl內標後,振盪混勻,正己烷1.0 ml萃取後離心,取上清液用N2吹榦,200μl無水乙醇複溶,UPLC法測定。色譜條件為色譜柱：Waters C18柱(2.1 mm ×100 mm,1.7μm)；二級陣列管檢測器；流動相：乙腈：水(94：6)臨用前脫氣；檢測波長325 nm；柱溫：40℃；流速：0.3 ml/min；進樣2μl。結果：360例學齡兒童血清VitA含量在0.0728~0.728μg/ml範圍內呈良好的線性關繫(r>0.999),VitA的平均值為39.60μg/dl,CV<5％,VitA的最低檢測限為0.020μg/ml,測定在4.0 min內完成。360例學齡兒童中VitA含量≤20μg/dl佔總人數的1.11％,VitA水平20~30μg/dl佔總人數的19.17％,VitA水平≥30μg/dl佔總人數的79.72％。結論：調查結果總體滿足膳食營養計劃的要求。反相UPLC內標法檢測兒童血清中VitA的水平,方法快速、靈敏、準確,重複性好,樣品處理簡單宜行。
목적：：건립반상초고효액상색보(UPLC)내표법측정인동혈청중유생소A(VitA)수평적방법,이료해학령인동VitA영양상황,위제정선식영양계화제공삼고。방법：부양시영상현학령인동100μl혈청가입100μl내표후,진탕혼균,정기완1.0 ml췌취후리심,취상청액용N2취간,200μl무수을순복용,UPLC법측정。색보조건위색보주：Waters C18주(2.1 mm ×100 mm,1.7μm)；이급진렬관검측기；류동상：을정：수(94：6)림용전탈기；검측파장325 nm；주온：40℃；류속：0.3 ml/min；진양2μl。결과：360례학령인동혈청VitA함량재0.0728~0.728μg/ml범위내정량호적선성관계(r>0.999),VitA적평균치위39.60μg/dl,CV<5％,VitA적최저검측한위0.020μg/ml,측정재4.0 min내완성。360례학령인동중VitA함량≤20μg/dl점총인수적1.11％,VitA수평20~30μg/dl점총인수적19.17％,VitA수평≥30μg/dl점총인수적79.72％。결론：조사결과총체만족선식영양계화적요구。반상UPLC내표법검측인동혈청중VitA적수평,방법쾌속、령민、준학,중복성호,양품처리간단의행。
Objective:To establish the reversed-phase ultra performance liquid chromatography( UPLC) with internal standard method for detecting the serum vitamin A level of children, in order to understand the school-age children vitamin A nutritional status, and provide reference in formulating the dietary nutrition plan. Methods:The 100μl standard was added to the 100 ml serum of school aged children in Fuyang city Yingshang county,which was oscillated,mixed,extracted using 1. 0 ml of hexane extraction and centrifugated. The supernatant was dried with N2 , redissoluting using 200 μl of anhydrous ethanol, and which was determined by UPLC. The chromatographic column was the chromatographic conditions,which included Waters C18 column(2. 1 mm × 100 mm,1. 7 μm), secondary array detector;mobile phase,acetonitrile:water(96:4) prodegassing before using. The detection wavelength was 325 nm,the column temperature was 40 ℃, the flow rate was 0. 3 ml/min and the adding sample was 2 μl. Results:The serum vitamin A concentration in 360 school-age children was 0. 072 8 to 0. 728 μg/ml,the range of which showed a good linear relationship(r >0. 999). The mean value of vitamin A was 39. 6 μg/dl,CV was less than 5%. The lowest detection limit of vitamin A was 0. 020 μg/ml,which could be detected within 4. 0 min. Among 360 school aged children,the children with vitamin A level equal to or less than 20 μg/dl,equal to 30 μg/dl and more than or equal to 30 μg/dl were accounted for 1. 11%,19. 17% and 79. 72%,respectively. Conclusions:The overall survey results meet the requirements of dietary nutrition plan. The reversed-phase ultra performance liquid chromatography with internal standard method in detecting the serum vitamin A level of children is fast, sensitive, accurate and reproducible.
