安徽医药
安徽醫藥
안휘의약
ANHUI MEDICAL AND PHARMACEUTICAL JOURNAL
2015年
9期
1661-1664
,共4页
乌头提取物%心力衰竭%抗氧化能力
烏頭提取物%心力衰竭%抗氧化能力
오두제취물%심력쇠갈%항양화능력
aconitum extract%heart failure%antioxidant capacity
目的：探讨乌头提取物对心衰大鼠抗氧化能力，并明确乌头提取物治疗心衰的作用。方法选取90只Wistar大鼠随机分为3组，空白组30只，对照组30只及实验组30只。实验组和对照组采用腹主动脉缩窄法制作慢性心衰大鼠模型。造模成功后对照组给予注射地高辛20 mg· kg－1，连续干预28 d；实验组在对照组的基础上加用乌头提取物，灌胃0．04 mg· kg－1，干预28 d，空白组以等体积生理盐水尾静脉注射0．04 g· kg－1。干预结束后，对大鼠采用腹腔注射麻醉方法，取腹主动脉血离心后取上层血清，按试剂盒方法测定超氧化物歧化酶（ SOD）、丙二醛（ MDA）、还原型谷胱甘肽（ GSH）。结果造模结束后，实验组与对照组SOD、MDA与GSH水平明显低于空白组，差异有统计学意义（ P＜0．05）。实验组与对照组SOD、MDA与GSH水平无明显差别，（ P＞0．05）；干预7 d及28 d后，与空白组相比，实验组与对照组SOD、MDA与GSH水平明显升高，差异有统计学意义（P＜0．05），与对照组相比，实验组SOD、MDA与GSH水平明显升高，差异有统计学意义（P＜0．05）。结论向心衰大鼠体内注射乌头提取物，对提高心衰机体的抗氧化能力、改善心功能不全指导意义，值得临床推广。
目的：探討烏頭提取物對心衰大鼠抗氧化能力，併明確烏頭提取物治療心衰的作用。方法選取90隻Wistar大鼠隨機分為3組，空白組30隻，對照組30隻及實驗組30隻。實驗組和對照組採用腹主動脈縮窄法製作慢性心衰大鼠模型。造模成功後對照組給予註射地高辛20 mg· kg－1，連續榦預28 d；實驗組在對照組的基礎上加用烏頭提取物，灌胃0．04 mg· kg－1，榦預28 d，空白組以等體積生理鹽水尾靜脈註射0．04 g· kg－1。榦預結束後，對大鼠採用腹腔註射痳醉方法，取腹主動脈血離心後取上層血清，按試劑盒方法測定超氧化物歧化酶（ SOD）、丙二醛（ MDA）、還原型穀胱甘肽（ GSH）。結果造模結束後，實驗組與對照組SOD、MDA與GSH水平明顯低于空白組，差異有統計學意義（ P＜0．05）。實驗組與對照組SOD、MDA與GSH水平無明顯差彆，（ P＞0．05）；榦預7 d及28 d後，與空白組相比，實驗組與對照組SOD、MDA與GSH水平明顯升高，差異有統計學意義（P＜0．05），與對照組相比，實驗組SOD、MDA與GSH水平明顯升高，差異有統計學意義（P＜0．05）。結論嚮心衰大鼠體內註射烏頭提取物，對提高心衰機體的抗氧化能力、改善心功能不全指導意義，值得臨床推廣。
목적：탐토오두제취물대심쇠대서항양화능력，병명학오두제취물치료심쇠적작용。방법선취90지Wistar대서수궤분위3조，공백조30지，대조조30지급실험조30지。실험조화대조조채용복주동맥축착법제작만성심쇠대서모형。조모성공후대조조급여주사지고신20 mg· kg－1，련속간예28 d；실험조재대조조적기출상가용오두제취물，관위0．04 mg· kg－1，간예28 d，공백조이등체적생리염수미정맥주사0．04 g· kg－1。간예결속후，대대서채용복강주사마취방법，취복주동맥혈리심후취상층혈청，안시제합방법측정초양화물기화매（ SOD）、병이철（ MDA）、환원형곡광감태（ GSH）。결과조모결속후，실험조여대조조SOD、MDA여GSH수평명현저우공백조，차이유통계학의의（ P＜0．05）。실험조여대조조SOD、MDA여GSH수평무명현차별，（ P＞0．05）；간예7 d급28 d후，여공백조상비，실험조여대조조SOD、MDA여GSH수평명현승고，차이유통계학의의（P＜0．05），여대조조상비，실험조SOD、MDA여GSH수평명현승고，차이유통계학의의（P＜0．05）。결론향심쇠대서체내주사오두제취물，대제고심쇠궤체적항양화능력、개선심공능불전지도의의，치득림상추엄。
Objective To investigate the effect of aconite extract on antioxidant capacity in rats with heart failure and the role of aconite extract in treatment of heart failure.Methods Ninety Wistar rats were randomized into three groups, blank control group(n=30), control group(n=30) and experimental group(n=30).The experimental group and the control group were abdominal aortic coarcta-tion rat model of chronic heart failure.After modeling the control group received injections of digoxin 20 mg· kg-1 , once a day for 28 days of continuous intervention, the experimental group had an additional use of aconite extract, administered 0.04 mg· kg-1 for an intervention of 28 days and the blank control group had tail vein injection of the same volume of satine at 0.04 g· kg-1 .After the end of the intervention, the rats were anesthetized by intraperitoneal injection, abdominal aortic blood taken from the upper serum after cen-trifugation.Kit method was used to measure superoxide dismutase ( SOD) , malondialdehyde ( MDA) and reduced glutathione ( GSH) . Results After the modelling, SOD, MDA and GSH levels in the experimental group and the control group were significantly lower than the blank control group with statistical difference (P<0.05).SOD, MDA and GSH levels in experimental group and control group were not significantly different (P>0.05).Seven days and 28 days after intervention, compared with the blank control group, SOD, MDA and GSH levels in the experimental group and control group were significantly increased with statistically difference ( P<0.05).Compared with the control group, SOD, MDA and GSH levels in the experimental group were significantly increased with sta-tistical difference (P <0.05).Conclusion Aconitum extract injected into the body of rats with heart failure can promote the body's antioxidant capacity and improve cardiac dysfunction, which is worthy of promotion.
