安徽医药
安徽醫藥
안휘의약
ANHUI MEDICAL AND PHARMACEUTICAL JOURNAL
2015年
9期
1652-1656
,共5页
骨髓干细胞%川芎嗪%修复%急性肾小管坏死
骨髓榦細胞%川芎嗪%脩複%急性腎小管壞死
골수간세포%천궁진%수복%급성신소관배사
mesenchymal stem cell%Ligustrazine%repair%acute tubuLar necrosis
目的:通过对于骨髓移植后甘油注射所致肾小管损伤小鼠使用川芎嗪,粒巨噬细胞集落刺激因子( GM-CSF ),观察促进骨髓基质细胞增殖的因素在骨髓干细胞对于肾小管损伤后参与修复的影响。方法绿色荧光蛋白标记( GFP)的C57BL/6转基因小鼠提供骨髓,同种无荧光标记的C57 BL/6小鼠分为生理盐水组( n=20)、20%川芎嗪干预组( n=20)、GM-CSF组干预组( n=20)进行血常规、肾功能、流式细胞仪以及肾脏病理检测,并采用荧光组织化学、免疫组织化学等方法观察绿色荧光在骨髓移植小鼠肾脏的分布及数量。结果经过骨髓移植以及甘油注射肾小管损伤后,三组在流式细胞仪结果中显示干预组GFP阳性细胞偏高(P<0.05),而病理结果显示:川芎嗪组病理评分结果较其他两组高(P<0.05),而GM-CSF有双向的表现。免疫组化显示干预组均有外源性的肾小管上皮细胞的增多( P<0.05)。结论川芎嗪和GM-CSF均可一定程度增强骨髓干细胞对肾小管损伤的修复作用。
目的:通過對于骨髓移植後甘油註射所緻腎小管損傷小鼠使用川芎嗪,粒巨噬細胞集落刺激因子( GM-CSF ),觀察促進骨髓基質細胞增殖的因素在骨髓榦細胞對于腎小管損傷後參與脩複的影響。方法綠色熒光蛋白標記( GFP)的C57BL/6轉基因小鼠提供骨髓,同種無熒光標記的C57 BL/6小鼠分為生理鹽水組( n=20)、20%川芎嗪榦預組( n=20)、GM-CSF組榦預組( n=20)進行血常規、腎功能、流式細胞儀以及腎髒病理檢測,併採用熒光組織化學、免疫組織化學等方法觀察綠色熒光在骨髓移植小鼠腎髒的分佈及數量。結果經過骨髓移植以及甘油註射腎小管損傷後,三組在流式細胞儀結果中顯示榦預組GFP暘性細胞偏高(P<0.05),而病理結果顯示:川芎嗪組病理評分結果較其他兩組高(P<0.05),而GM-CSF有雙嚮的錶現。免疫組化顯示榦預組均有外源性的腎小管上皮細胞的增多( P<0.05)。結論川芎嗪和GM-CSF均可一定程度增彊骨髓榦細胞對腎小管損傷的脩複作用。
목적:통과대우골수이식후감유주사소치신소관손상소서사용천궁진,립거서세포집락자격인자( GM-CSF ),관찰촉진골수기질세포증식적인소재골수간세포대우신소관손상후삼여수복적영향。방법록색형광단백표기( GFP)적C57BL/6전기인소서제공골수,동충무형광표기적C57 BL/6소서분위생리염수조( n=20)、20%천궁진간예조( n=20)、GM-CSF조간예조( n=20)진행혈상규、신공능、류식세포의이급신장병리검측,병채용형광조직화학、면역조직화학등방법관찰록색형광재골수이식소서신장적분포급수량。결과경과골수이식이급감유주사신소관손상후,삼조재류식세포의결과중현시간예조GFP양성세포편고(P<0.05),이병리결과현시:천궁진조병리평분결과교기타량조고(P<0.05),이GM-CSF유쌍향적표현。면역조화현시간예조균유외원성적신소관상피세포적증다( P<0.05)。결론천궁진화GM-CSF균가일정정도증강골수간세포대신소관손상적수복작용。
Objective To advanced demonstrate bone-marrow-derived mesenchymal stem cells can differentiate into renal tubular epi-thelial cells and discuss the factor of accelerating hematopoietic reconstitution effect on the repair of tubular injury by bone marrow transplantion mice perform glycerol injection.Methods Transgenic C57BL/6 mice labeled with GFP as bone maeeow donors,C57BL/6 mice,recipients of bone marrow transplantion,were divided into three groups:(1)normal saline group;(2)20%Ligustrazine group, (3) GM-CSF group.All groups procee blood rountine,renal function,flow cytometry of bone marrow cells,and each side kidney histolo-gy,distribution and amount of green fluorescence in kidney of recipient mice observed by fluorescence histochemistry, immunohisto-chemistry means.Results Flow cytometry of bone marrow cells show the 2,3 groups more increase than the 1 group,the 2 group can enhance the recovery of renal injury,the 3 group show a“two-way”influence in the recovery of renal injury.In the fluorescence histo-chemistry and immunohistochemistry,the 2,3 groups show have a few increase in the number of ectogenesis( GFP+) renal tubular epi-thelial cells.Conclusion Ligustrazine or GM-CSF can enhanced the repair of tubuLar injury in glycerol injection injury mice by bone-marrow-derived mesenchymal stem cell.