蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
8期
994-997,1001
,共5页
肺肿瘤%白藜芦醇%紫杉醇%凋亡
肺腫瘤%白藜蘆醇%紫杉醇%凋亡
폐종류%백려호순%자삼순%조망
lung neoplasms%resveratrol%paclitaxel%apoptosis
目的：：探讨白藜芦醇( RES)联合紫杉醇( PTX)对人肺癌细胞PC9的凋亡作用及其机制。方法：不同浓度的RES和PTX单用和联合作用于PC9细胞后,分别采用四甲基偶氮唑蓝法测定其对PC9的增殖抑制作用,流式细胞术检测其对细胞周期分布和凋亡的影响,Western blot法检测Caspase-3蛋白、抗凋亡蛋白Bcl-2、促凋亡Bax表达的变化。结果：10、20、30μmol/L的RES和0.001、0.01、0.1μmol/L的PTX均以时间和浓度依赖方式抑制PC9细胞的增殖,RES联合PTX的抑制率高于RES和PTX单用组(P<0.01)。单用RES和PTX均能诱导PC9细胞发生凋亡和G0/G1期阻滞,两者联合应用,诱导细胞凋亡和G0/G1期阻滞作用显著增强(P<0.01)。 Western blot法检测显示在RES和PTX联用之后,Caspase-3蛋白、Bcl-2、Bax的改变显著大于单药作用。结论：RES、PTX均可抑制人肺癌PC9细胞增殖、诱导其凋亡、阻滞细胞G0/G1期、伴随Caspase-3活性上调,且两者联合应用具有协同作用,可显著促进细胞凋亡,其机制可能与上调凋亡蛋白Bcl-2、下调抗凋亡蛋白Bax有关。
目的：：探討白藜蘆醇( RES)聯閤紫杉醇( PTX)對人肺癌細胞PC9的凋亡作用及其機製。方法：不同濃度的RES和PTX單用和聯閤作用于PC9細胞後,分彆採用四甲基偶氮唑藍法測定其對PC9的增殖抑製作用,流式細胞術檢測其對細胞週期分佈和凋亡的影響,Western blot法檢測Caspase-3蛋白、抗凋亡蛋白Bcl-2、促凋亡Bax錶達的變化。結果：10、20、30μmol/L的RES和0.001、0.01、0.1μmol/L的PTX均以時間和濃度依賴方式抑製PC9細胞的增殖,RES聯閤PTX的抑製率高于RES和PTX單用組(P<0.01)。單用RES和PTX均能誘導PC9細胞髮生凋亡和G0/G1期阻滯,兩者聯閤應用,誘導細胞凋亡和G0/G1期阻滯作用顯著增彊(P<0.01)。 Western blot法檢測顯示在RES和PTX聯用之後,Caspase-3蛋白、Bcl-2、Bax的改變顯著大于單藥作用。結論：RES、PTX均可抑製人肺癌PC9細胞增殖、誘導其凋亡、阻滯細胞G0/G1期、伴隨Caspase-3活性上調,且兩者聯閤應用具有協同作用,可顯著促進細胞凋亡,其機製可能與上調凋亡蛋白Bcl-2、下調抗凋亡蛋白Bax有關。
목적：：탐토백려호순( RES)연합자삼순( PTX)대인폐암세포PC9적조망작용급기궤제。방법：불동농도적RES화PTX단용화연합작용우PC9세포후,분별채용사갑기우담서람법측정기대PC9적증식억제작용,류식세포술검측기대세포주기분포화조망적영향,Western blot법검측Caspase-3단백、항조망단백Bcl-2、촉조망Bax표체적변화。결과：10、20、30μmol/L적RES화0.001、0.01、0.1μmol/L적PTX균이시간화농도의뢰방식억제PC9세포적증식,RES연합PTX적억제솔고우RES화PTX단용조(P<0.01)。단용RES화PTX균능유도PC9세포발생조망화G0/G1기조체,량자연합응용,유도세포조망화G0/G1기조체작용현저증강(P<0.01)。 Western blot법검측현시재RES화PTX련용지후,Caspase-3단백、Bcl-2、Bax적개변현저대우단약작용。결론：RES、PTX균가억제인폐암PC9세포증식、유도기조망、조체세포G0/G1기、반수Caspase-3활성상조,차량자연합응용구유협동작용,가현저촉진세포조망,기궤제가능여상조조망단백Bcl-2、하조항조망단백Bax유관。
Objective:To explore the apoptosis-inducing effects of the application of the resveratrol(RES)combined with paclitaxel (PTX)on lung cancer cell line PC9,and its proable mechanism. Methods:After the PC9 cells were co-cultured with the single and combination of different concentrations two drugs, the anti-proliferative effects of drugs were measured using MTT, the cell cycle distribution and apoptosis were detected by flow cytometry,and the protein levels of Caspase-3,Bcl-2 and Bax were detected by western blotting. Results:The effects of RES and PTX on the inhibiting the prolification of PC9 cells were the time-and concentrate-dependent manner. The inhibition rate of RES combined with paclitaxel was higher than that of single RES or PTX use(P<0. 01). The single use of RES or PTX could induce the apoptosis and block G0/G1 phase in PC9 cells,and the effect of which was significantly enhanced when two drugs were combined(P<0. 01). Western blot assay showed that the changes of Caspase-3,Bcl-2 and Bax after the joint use of RES and PTX were significantly more than that of the single drug use. Conclusions:Both RES and PTX can inhibit the proliferation, induce apoptosis,block G0/G1 phase and increase the Caspase-3 activity of human lung cancer PC9 cells. The combined use of RES and PTX has synergistic effect,which can significantly promote the cell apoptosis,and the mechanism may be related to the upregulation of Bcl-2 and downregulation of Bax.
