CAJ | 학술논문

目的：研究原花青素对微小RNA-27a（miR-27a）与微小RNA-451（miR-451）在A2780/T细胞中表达的影响，及其与抑制多药耐药基因1（MDR1） mRNA表达的相关性。方法通过茎环法聚合酶链反应（stem-loop PCR），检测miR-27a与miR-451的表达水平，分别评价0~40μmol/L的原花青素刺激A2780/T细胞0~48 h后细胞内miR-27a与miR-451的表达水平。进一步通过转染miR-27a和miR-451的模拟或抑制片段，分别过表达或沉默相关的微小RNA，采用RT-PCR法评价原花青素对转染后细胞内MDR1 mRNA表达水平的影响。结果原花青素显著抑制了miR-27a与miR-451的表达水平，并且均具有浓度与时间依赖性。原花青素能够显著抑制过表达miR-27a与miR-451而上调的MDR1 mRNA水平，而对通过miR-27a与miR-451的沉默而下调的MDR1 mRNA水平无显著影响。结论原花青素能够通过抑制A2780/T细胞内miR-27a与miR-451的表达，从而抑制MDR1 mRNA的表达。
목적：연구원화청소대미소RNA-27a（miR-27a）여미소RNA-451（miR-451）재A2780/T세포중표체적영향，급기여억제다약내약기인1（MDR1） mRNA표체적상관성。방법통과경배법취합매련반응（stem-loop PCR），검측miR-27a여miR-451적표체수평，분별평개0~40μmol/L적원화청소자격A2780/T세포0~48 h후세포내miR-27a여miR-451적표체수평。진일보통과전염miR-27a화miR-451적모의혹억제편단，분별과표체혹침묵상관적미소RNA，채용RT-PCR법평개원화청소대전염후세포내MDR1 mRNA표체수평적영향。결과원화청소현저억제료miR-27a여miR-451적표체수평，병차균구유농도여시간의뢰성。원화청소능구현저억제과표체miR-27a여miR-451이상조적MDR1 mRNA수평，이대통과miR-27a여miR-451적침묵이하조적MDR1 mRNA수평무현저영향。결론원화청소능구통과억제A2780/T세포내miR-27a여miR-451적표체，종이억제MDR1 mRNA적표체。
Objective To investigate the effect of expression of microRNA-27a(miR-27a) and microRNA-451(miR-451) in A2780/T cells and its relativity to multidrug resistance (MDR)1 mRNA inhibition by procyanidin. Methods Stem-loop PCR method was performed to evaluate the expression of miR-27a and miR-451 in use of procyanidin (0-40μmol/L) in 0-48 h in A2780/T cells. Additionally, over-expressing or interfecting microRNAs by using mimics or inhibitor of miR-27a and miR-451, the expression of MDR1 mRNA was assessed by RT-PCR in cells exposing to procyanidin. Results The expression of miR-27a and miR-451 was significant inhibited by procyanidin in both time- and concentration-dependency. Over-expressed MDR1 mRNA associated with miR-27a or miR-451 mimics was blocked by procyanidin, whereas there was no effect on down-expressed MDR1 mRNA associated with miR-27a or miR-451 inhibitor by procyanidin. Conclusion Procyanidin inhibits MDR1 mRNA expression by inhibiting miR-27a and miR-451 expression in A2780/T cells.