CAJ | 학술논문

目的： HPLC法建立苍柏祛痛胶囊镇痛活性组分的指纹特征图谱，对不同生产批号苍柏祛痛胶囊进行质量控制。方法采用Waters XSELECT CSH-C18（4.6 mm×150 mm，5μm）色谱柱，以乙腈-0.1%磷酸溶液梯度洗脱，流速为1.0 ml/min，柱温30℃，检测波长为284 nm。以盐酸黄柏碱、龙胆苦苷、芍药苷、粉防己碱和盐酸小檗碱为参照物，对10批苍柏祛痛胶囊进行了相似度分析。结果苍柏祛痛胶囊特征图谱中共有20个共有峰，并在其中对出黄柏、秦艽、赤芍和防己的特征峰，各批样品的相似度均＞0.9。结论采用HPLC特征图谱可实现全面和整体的评价，为有效提高苍柏祛痛胶囊的质量控制提供参考。
목적： HPLC법건립창백거통효낭진통활성조분적지문특정도보，대불동생산비호창백거통효낭진행질량공제。방법채용Waters XSELECT CSH-C18（4.6 mm×150 mm，5μm）색보주，이을정-0.1%린산용액제도세탈，류속위1.0 ml/min，주온30℃，검측파장위284 nm。이염산황백감、룡담고감、작약감、분방기감화염산소벽감위삼조물，대10비창백거통효낭진행료상사도분석。결과창백거통효낭특정도보중공유20개공유봉，병재기중대출황백、진구、적작화방기적특정봉，각비양품적상사도균＞0.9。결론채용HPLC특정도보가실현전면화정체적평개，위유효제고창백거통효낭적질량공제제공삼고。
Objective To establish fingerprint of analgesic ingredients in Cangbaiqutong capsules by HPLC, and control different production batches of Cangbaiqutong capsules′ quality. Methods Waters XSELECT CSH-C18 (4.6 mm×150 mm, 5.0μm) chromatographic column was used and eluted with acetonitrile -0.1% phosphoric acid solution gradiently in the flow rate of 1.0 ml/min. The detection wavelength was 284 nm with column temperature of 30℃. rrith phellodendrine chloride, gentiopicroside, paeoniflorin, tetrandrine and berberine hydrochloride as the object references, ten batches of Cangbaiqutong capsules were tested and analyzed by similarity comparison. Results fingerprint spectrum in Cangbaiqutong capsules had 20 common peaks in total, and characteristic spectra of Cortex Phellodendri, Gentiana, Radix Paeoniae Rubra and Radix Stephaniae Tetrandrae were found, while similarity of HPLC fingerprint was more than 0.9 among those batches of samples. Conclusion Using HPLC fingerprint can be used to evaluate the Cangbaiqutong capsules′quality in the round, which could improve the quality control standard in productive process of Cangbaiqutong capsules.