CAJ | 학술논문

目的：建立含麦芽糊精的枸杞提取物干粉中多糖含量的测定方法。方法采用糖化酶水解麦芽糊精，醇沉后苯酚硫酸法测定多糖含量，优化糖化酶水解麦芽糊精的工艺，比较酶解麦芽糊精后测定多糖含量、直接测定多糖含量与样品中多糖含量真实值之间的差异。并考察糖化酶对枸杞多糖的水解能力。结果糖化酶水解麦芽糊精的最佳工艺条件为：酶解温度60℃、酶与底物比例为1∶5，反应时间为30 min。与样品中枸杞多糖含量的真实值相比，直接测定值显著偏高，组间比较数据有显著统计学差异（P＜0.01），酶解麦芽糊精后测定值无显著性差异（P＞0.05）；与直接测定值相比，酶解麦芽糊精后测定值显著降低（P＜0.01），且糖化酶对枸杞多糖无水解能力。结论糖化酶水解法可消除麦芽糊精对枸杞提取物干粉中多糖测定的影响，且方法简单、有效。
목적：건립함맥아호정적구기제취물간분중다당함량적측정방법。방법채용당화매수해맥아호정，순침후분분류산법측정다당함량，우화당화매수해맥아호정적공예，비교매해맥아호정후측정다당함량、직접측정다당함량여양품중다당함량진실치지간적차이。병고찰당화매대구기다당적수해능력。결과당화매수해맥아호정적최가공예조건위：매해온도60℃、매여저물비례위1∶5，반응시간위30 min。여양품중구기다당함량적진실치상비，직접측정치현저편고，조간비교수거유현저통계학차이（P＜0.01），매해맥아호정후측정치무현저성차이（P＞0.05）；여직접측정치상비，매해맥아호정후측정치현저강저（P＜0.01），차당화매대구기다당무수해능력。결론당화매수해법가소제맥아호정대구기제취물간분중다당측정적영향，차방법간단、유효。
Objective To establish a method for determination of polysaccharide in Lycium barbarum L . extract powder containing with malt dextrin. Method Maltodextrin was treated with glucoamylase hydrolysis and ethanol precipitation successively;then the phenol-sulfuric acid method was used to determine the content of polysaccharide. The glucoamylase hydrolysis technolgy of malt dextrin was optimized, and the variation of polysaccharide content before and after hydrolysis, the difference of polysaccharide contents between direct determination and truth were compared. Morever, the hydrolysis effect of glucoamylase on Lycium barbarum L. was investigated. Results The optimum hydrolysis condition was: temperature 60℃, the ratio of enzyme to substrate 1∶5, hydrolysis time 30 min. The polysaccharide content determined directly was significantly higher than the real value (P<0.01); the polysaccharide content determined after hydrolysis decreased significantly (P<0.01) but showed no significant difference with the real value (P>0.05). Glucoamylase showed no hydrolysis effects on Lycium barbarum L. polysaccharide. Conclusion Glucoamylase hydrolysis can deplete the influence of malt dextrin on determination of polysaccharide in Lycium barbarum L. extract powder, the procedure is simple and effective.