CAJ | 학술논문

为研究多级水提取对造纸法再造烟叶原料中有机酸的提取效果，采用气相色谱-质谱联用法测定了碎烟片（简称烟片）原料和各级滤渣中主要挥发性、半挥发性和非挥发性有机酸的含量(质量分数)。结果表明：①原料和各级滤渣中有机酸的含量存在明显差异，随着提取级数的增加，滤渣中挥发性和非挥发性有机酸总量减小，半挥发性有机酸的总量增加。原料和滤渣中的有机酸以草酸、苹果酸和柠檬酸为主，3种酸总量随提取级数的增加而减小。②滤渣中半挥发性有机酸和草酸的含量均高于其在烟片原料中的含量。采用多级水提取方法有利于烟叶原料中多数有机酸成分的提取，但并不能将烟片原料中半挥发性有机酸和草酸有效提取出来。
위연구다급수제취대조지법재조연협원료중유궤산적제취효과，채용기상색보-질보련용법측정료쇄연편（간칭연편）원료화각급려사중주요휘발성、반휘발성화비휘발성유궤산적함량(질량분수)。결과표명：①원료화각급려사중유궤산적함량존재명현차이，수착제취급수적증가，려사중휘발성화비휘발성유궤산총량감소，반휘발성유궤산적총량증가。원료화려사중적유궤산이초산、평과산화저몽산위주，3충산총량수제취급수적증가이감소。②려사중반휘발성유궤산화초산적함량균고우기재연편원료중적함량。채용다급수제취방법유리우연협원료중다수유궤산성분적제취，단병불능장연편원료중반휘발성유궤산화초산유효제취출래。
In order to investigate the effect of multi-stage water extraction on organic acids extracting from raw material of paper-making process reconstituted tobacco, the contents of major volatile, semi-volatile, and nonvolatile organic acids in tobacco and residues from each stage were determined by gas chromatography-mass spectrometry (GC/MS). The results showed that: 1) The contents of volatile, semi-volatile, and nonvolatile organic acids in tobacco and residues were significantly different. Generally, with the increase of number of extraction stage, the total amounts of volatile and non-volatile organic acids in residues decreased, while the total amount of semi-volatile organic acids increased. The major organic acids in tobacco and residues were oxalic acid, malic acid and citric acid, and their total amount decreased with the increase of number of extraction stage. 2) The contents of semi-volatile organic acids and oxalic acid in residues were higher than those in tobacco. Multi-stage water extraction is effective for the extraction of most organic acids in tobacco, but it is not effective for extracting semi-volatile organic acids and oxalic acid from tobacco.