CAJ | 학술논문

通过水热法一步合成了纳米Fe3 O4微球，并在甲苯中用巯基丙基三甲氧基硅烷（M PS ）对其进行表面修饰得到了Fe3 O4‐SH微球，通过DTNB法测得微球表面巯基含量为3335．4μg／mg ．该纳米微球可以吸附溶液中的Ni2＋，从而形成Fe3 O4‐SH‐Ni2＋复合材料．以此复合材料为载体，可以将以组氨酸为标签的（His‐tagged）融合蛋白直接从细胞裂解液中进行提纯，并在外加磁场的作用下实现对目标蛋白的快速分离，其对 His‐tagged TRX蛋白的分离能力为206．μg／m g ，特别适合于对以组氨酸为标签蛋白的分离纯化．
통과수열법일보합성료납미Fe3 O4미구，병재갑분중용구기병기삼갑양기규완（M PS ）대기진행표면수식득도료Fe3 O4‐SH미구，통과DTNB법측득미구표면구기함량위3335．4μg／mg ．해납미미구가이흡부용액중적Ni2＋，종이형성Fe3 O4‐SH‐Ni2＋복합재료．이차복합재료위재체，가이장이조안산위표첨적（His‐tagged）융합단백직접종세포렬해액중진행제순，병재외가자장적작용하실현대목표단백적쾌속분리，기대 His‐tagged TRX단백적분리능력위206．μg／m g ，특별괄합우대이조안산위표첨단백적분리순화．
Ferriferrous oxide magnetic microspheres (MSs) were prepared via solvothermal route .The so‐obtained MSs were modified by (3‐mercapto‐propyl)trimethoxysilane (MPS) in mythylbenzene to give birth of the ferriferrous oxide with thiol group (Fe3 O4‐SH ) micro‐spheres ,which can absorb Ni2+ to form Fe3 O4‐SH‐Ni2+ .The amount of thiol group of Fe3 O4‐SH was tested to be 333 5.4 μg/mg by means of DTNB method .After chelating Ni2+ ions ,Fe3 SH‐Ni2+ MSs were used to enrich and purify histidine‐tagged (His‐tagged) proteins directly from the mixture of lysed cells without pretreatment .Moreover ,the prepared MSs can sepa‐rate the target protein quickly in the magnetic field .It has been found that Fe3 O4‐SH‐Ni2+ MSs present negligible nonspecific protein adsorption and high protein binding activity with the sat‐uration capacity being 20 6.μg/mg and they are especially suitable for rapid purification of His‐tagged proteins .