CAJ | 학술논문

目的：：对他巴唑对甲状腺滤泡细胞的影响进行深入探讨和剖析，并对其作用的构效关系进行研究，以供相关工作人员借鉴。方法：用不同浓度的他巴唑对培养过的甲状腺滤泡细胞(TFC)在不同作用时间进行比较，另外，用类似结构的药物，比如硫脲、咪唑处理甲状腺滤泡细胞，应用 Annexin-V FITC/PI 双标染流式细胞仪对甲状腺滤泡细胞凋亡率进行检测，对比研究他巴唑对甲状腺滤泡细胞凋亡的影响。结果：不同剂量的他巴唑对甲状腺滤泡细胞凋亡的诱导有依赖性，甲状腺滤泡细胞数量的明显增多就是证明。类似药物中的硫脲对甲状腺滤泡细胞有相似的效果，但是咪唑对甲状腺滤泡细胞没有相关的效果。结论：他巴唑增多甲状腺滤泡细胞的数量比率，主要是依赖硫脲基因产生诱导凋亡作用的。
목적：：대타파서대갑상선려포세포적영향진행심입탐토화부석，병대기작용적구효관계진행연구，이공상관공작인원차감。방법：용불동농도적타파서대배양과적갑상선려포세포(TFC)재불동작용시간진행비교，령외，용유사결구적약물，비여류뇨、미서처리갑상선려포세포，응용 Annexin-V FITC/PI 쌍표염류식세포의대갑상선려포세포조망솔진행검측，대비연구타파서대갑상선려포세포조망적영향。결과：불동제량적타파서대갑상선려포세포조망적유도유의뢰성，갑상선려포세포수량적명현증다취시증명。유사약물중적류뇨대갑상선려포세포유상사적효과，단시미서대갑상선려포세포몰유상관적효과。결론：타파서증다갑상선려포세포적수량비솔，주요시의뢰류뇨기인산생유도조망작용적。
Objective:Discuss and analyze the influences of tapazole on thyroid follicular cells (TFC)and study the struc-ture-activity relationship of the influences,so as to provide a certain reference to the related personnel.Methods:Compare TFC cultured with tapazole of varied concentration at different time,treat TFC with drugs of the similar structure,including thioure-a,imidazole,test the TFC apoptosis rate with the Annexin-V FITC/PI Dou S-P flow cytometer,and make a comparative re-search on the influences of tapazole on the TFC apotosis.Results:Tapazole of different dosages has induction and dependence on the TFC apotosis,which is justified by obvious increase of TFC.Thiourea in similar drugs has the similar effect on TFC, but imidazole has not the related effect on TFC.Conclusion:Topazole produces apoptosis effect of induced thyroid filtration cell by increasing the mumber rate of the thyroid filtration cells,aiming at the thioureido group.