中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2015年
22期
56-57
,共2页
降纤酶%磷酸三丁酯%残留量%气相色谱法%测定
降纖酶%燐痠三丁酯%殘留量%氣相色譜法%測定
강섬매%린산삼정지%잔류량%기상색보법%측정
Deifbrase%Tri(n-butyl) phosphate(TNBP)%Residue%Gas chromatography%Assay
目的：建立毛细管气相色谱法测定降纤酶原液中磷酸三丁酯残留量。方法以磷酸三丙酯作内标，采用AT-FFAP色谱柱(30 m×0.32μm×0.25 mm)；气化室温度190℃；检测器温度210℃；柱温140℃；载气为氮气。结果磷酸三丁酯在质量浓度2~16μg/mL内线性关系良好；r为0.9973；质控样品在低、中、高3个浓度下加样回收率分别为98%、101.3%、100.6%，RSD分别为：1.8%、1.08%、2.51%(n=3)。结论该方法快速、准确，适用于降纤酶原液中磷酸三丁酯残留量的测定。
目的：建立毛細管氣相色譜法測定降纖酶原液中燐痠三丁酯殘留量。方法以燐痠三丙酯作內標，採用AT-FFAP色譜柱(30 m×0.32μm×0.25 mm)；氣化室溫度190℃；檢測器溫度210℃；柱溫140℃；載氣為氮氣。結果燐痠三丁酯在質量濃度2~16μg/mL內線性關繫良好；r為0.9973；質控樣品在低、中、高3箇濃度下加樣迴收率分彆為98%、101.3%、100.6%，RSD分彆為：1.8%、1.08%、2.51%(n=3)。結論該方法快速、準確，適用于降纖酶原液中燐痠三丁酯殘留量的測定。
목적：건립모세관기상색보법측정강섬매원액중린산삼정지잔류량。방법이린산삼병지작내표，채용AT-FFAP색보주(30 m×0.32μm×0.25 mm)；기화실온도190℃；검측기온도210℃；주온140℃；재기위담기。결과린산삼정지재질량농도2~16μg/mL내선성관계량호；r위0.9973；질공양품재저、중、고3개농도하가양회수솔분별위98%、101.3%、100.6%，RSD분별위：1.8%、1.08%、2.51%(n=3)。결론해방법쾌속、준학，괄용우강섬매원액중린산삼정지잔류량적측정。
Objective To establish a capillary gas chromatography (GC) for the determination of residue tri(n-butyl) phosphate (TNBP) in the original solution of deifbrase. Methods Using tripropyl phosphate (TPP) as internal standard, a simple capillary GC method was developed with a AT-FFAP capillary column (30 m×0.32 μm×0.25 mm);the injection port temperature was 190℃;the detector temperature was 210℃;column temperature was 140℃;the carries gas was high purity nitrogen. Results The linear range of TNBP was 2-16 μg/mL(r=0.9973). At low, medium and high concentrations, the recovery of TNBP was 98%, 101.3%, 100.6%respectively, and the RSD was 1.8%, 1.08%and 2.51%(n=3) respectively. Conclusion The methods is simple, precise and accurate, and can be used to determine residue of TNBP in the original solution of deifbrase.
