CAJ | 학술논문

目的：研究杨梅黄酮对炎性痛大鼠的外周镇痛作用及机制。方法采用大鼠左侧后肢足底中心皮下注射完全弗氏佐剂(complete Freund′s adjuvant, CFA)建立慢性炎性痛模型。检测给予杨梅黄酮前后大鼠热缩足反射潜伏期( ther-mal withdrawal latency,TWL)的改变。电生理学方法分析杨梅黄酮对背根节神经元放电频率及电压依赖性钾电流的影响。结果大鼠左侧足底皮下注射CFA 致炎后,TWL 明显降低( P <0．05),腹腔注射50、500 mg · kg-1杨梅黄酮使CFA组大鼠TWL 明显升高( P<0．05),电流钳记录显示杨梅黄酮抑制背根节小神经元的放电频率(P<0．01),且该抑制作用是通过增加钙依赖性钾通道电流( P<0．05)产生的。结论杨梅黄酮通过增加背根节神经元钙依赖性钾电流,抑制神经元兴奋性而发挥外周镇痛作用。
목적：연구양매황동대염성통대서적외주진통작용급궤제。방법채용대서좌측후지족저중심피하주사완전불씨좌제(complete Freund′s adjuvant, CFA)건립만성염성통모형。검측급여양매황동전후대서열축족반사잠복기( ther-mal withdrawal latency,TWL)적개변。전생이학방법분석양매황동대배근절신경원방전빈솔급전압의뢰성갑전류적영향。결과대서좌측족저피하주사CFA 치염후,TWL 명현강저( P <0．05),복강주사50、500 mg · kg-1양매황동사CFA조대서TWL 명현승고( P<0．05),전류겸기록현시양매황동억제배근절소신경원적방전빈솔(P<0．01),차해억제작용시통과증가개의뢰성갑통도전류( P<0．05)산생적。결론양매황동통과증가배근절신경원개의뢰성갑전류,억제신경원흥강성이발휘외주진통작용。
Aim To investigate the periphery analge-sic effect of myricetin on a rat model of inflammatory pain and the mechanism. Methods Rat models of in-flammatory pain were induced by complete Freund ’ s adjuvant ( CFA) injection in left hindlimb plantar cen-ter. The thermal withdrawal latency ( TWL) was meas-ured before and after CFA or myricetin treatment. Elec-trophysiological method was used to identify the effect of myricetin on the action potential frequency and the voltage dependent potassium channel currents in small DRG neurons. Results Rats with CFA injected showed thermal hyperalgesia ( P <0. 05 ) and TWL in-creased significantly after myricetin intraperitoneally in-jected ( P <0. 05 ) . Current clamp recording showed the action potential frequency of small DRG neurons in rats was inhibited by myricetin ( P<0. 01 ) and voltage calmap recording showed the inhibitory effect of myr-icetin was enhanced by calcium depended potassium channel currents ( P<0. 05 ) . Conclusion Myricetin exerts periphery analgesic effect by enhancing calcium depended potassium channel currents and inhibiting excitability of small neurons of dorsal root ganglion.