中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
8期
1142-1146
,共5页
补体%补体旁路活化%内皮细胞%纤溶%凝血%PDTC%白藜芦醇
補體%補體徬路活化%內皮細胞%纖溶%凝血%PDTC%白藜蘆醇
보체%보체방로활화%내피세포%섬용%응혈%PDTC%백려호순
complement%activation of the complement alternative pathway%endothelial cells%fibrinolysis%co-agulation%PDTC%resveratrol
目的：研究补体旁路激活致微血管内皮细胞纤溶凝血相关分子表达的变化及干预。方法采用眼镜蛇毒因子激活血清补体旁路途径。将旁路活化的血清作用于人微血管内皮细胞,通过ELISA检测多个时间点细胞培养上清中P-selectin、VWF、t-PA、PAI-1、TF、TM 的表达,采用 NO 试剂盒检测NO水平,并进一步研究吡咯烷二硫氨基甲酸( PDTC)、白藜芦醇对以上指标变化的影响。结果微血管内皮细胞经补体旁路活化产物刺激后,P-selectin、VWF的表达快速上调,其高峰时间为15 min,而纤溶功能分子t-PA、PAI-1也随后出现持续上调表达,与凝血功能相关的组织因子TF的表达水平持续上调,而血栓蛋白 TM 及 NO 的表达下降。PDTC、白藜芦醇对P-selectin、VWF、t-PA、PAI-1、TF上调表达具有抑制作用,对NO下调表达具有干预作用。而白藜芦醇能使TM的表达进一步下调。结论补体旁路活化产物会导致微血管内皮细胞纤溶凝血功能相关分子表达的变化,而PDTC、白藜芦醇对此种变化具有一定的影响。
目的：研究補體徬路激活緻微血管內皮細胞纖溶凝血相關分子錶達的變化及榦預。方法採用眼鏡蛇毒因子激活血清補體徬路途徑。將徬路活化的血清作用于人微血管內皮細胞,通過ELISA檢測多箇時間點細胞培養上清中P-selectin、VWF、t-PA、PAI-1、TF、TM 的錶達,採用 NO 試劑盒檢測NO水平,併進一步研究吡咯烷二硫氨基甲痠( PDTC)、白藜蘆醇對以上指標變化的影響。結果微血管內皮細胞經補體徬路活化產物刺激後,P-selectin、VWF的錶達快速上調,其高峰時間為15 min,而纖溶功能分子t-PA、PAI-1也隨後齣現持續上調錶達,與凝血功能相關的組織因子TF的錶達水平持續上調,而血栓蛋白 TM 及 NO 的錶達下降。PDTC、白藜蘆醇對P-selectin、VWF、t-PA、PAI-1、TF上調錶達具有抑製作用,對NO下調錶達具有榦預作用。而白藜蘆醇能使TM的錶達進一步下調。結論補體徬路活化產物會導緻微血管內皮細胞纖溶凝血功能相關分子錶達的變化,而PDTC、白藜蘆醇對此種變化具有一定的影響。
목적：연구보체방로격활치미혈관내피세포섬용응혈상관분자표체적변화급간예。방법채용안경사독인자격활혈청보체방로도경。장방로활화적혈청작용우인미혈관내피세포,통과ELISA검측다개시간점세포배양상청중P-selectin、VWF、t-PA、PAI-1、TF、TM 적표체,채용 NO 시제합검측NO수평,병진일보연구필각완이류안기갑산( PDTC)、백려호순대이상지표변화적영향。결과미혈관내피세포경보체방로활화산물자격후,P-selectin、VWF적표체쾌속상조,기고봉시간위15 min,이섬용공능분자t-PA、PAI-1야수후출현지속상조표체,여응혈공능상관적조직인자TF적표체수평지속상조,이혈전단백 TM 급 NO 적표체하강。PDTC、백려호순대P-selectin、VWF、t-PA、PAI-1、TF상조표체구유억제작용,대NO하조표체구유간예작용。이백려호순능사TM적표체진일보하조。결론보체방로활화산물회도치미혈관내피세포섬용응혈공능상관분자표체적변화,이PDTC、백려호순대차충변화구유일정적영향。
Aim To investigate the change of molecu-lar expression related to coagulation and fibrinolysis in human microvascular endothelial cells ( HMEC ) in-duced by activated complement alternative pathway and effect of pyrrolidine dithiocarbamate ( PDTC ) and res-veratrol on intervention. Methods Normal human se-rum was activated by cobra venom factor ( CVF) . After exposure of HMEC to activated complement for various times, supernatant was removed and assayed for ex-pressions of P-selectin, VWF, t-PA, PAI-1, TF, TM, and NO by using reagent kits. The expressions of the above molecules in HMEC pretreated with PDTC and resveratrol were also investigated. Results P-selectin and VWF were rapidly released by endothelial cells and the expression reached the peak at the time point of 15 min. The expressions of t-PA, PAI-1, and TF were continuously upregulated, whereas NO and TM were decreased. PDTC and resveratrol inhibited the upregulation of P-selectin, VWF, t-PA, PAI-1 and TF, and intervened the downregulation of NO. Res-veratrol further downregulated the expression of TM. Conclusion Activated complement alternative path-way can influence the expression of molecules related to coagulation and fibrinolysis in HMEC, and PDTC and resveratrol can affect this change.
