CAJ | 학술논문

目的：观察螺旋藻多糖对慢性乙型肝炎患者外周血单个核细胞免疫功能的影响。方法运用MTT法和流式细胞术分别测定螺旋藻多糖对慢性乙型肝炎患者外周血单个核细胞增殖能力和细胞周期的影响,采用ELISA法检测外周血单个核细胞培养上清细胞因子IFN-γ、IL-2和IL-4浓度,同时用RT-PCR法检测外周血单个核细胞 IFN-γ mRNA的表达。结果螺旋藻多糖能增强慢性乙型肝炎患者外周血单个核细胞的增殖能力,能增强慢性乙型肝炎患者外周血单个核细胞分泌IFN-γ和IL-2,且能降低分泌IL-4。螺旋藻多糖能上调慢性乙型肝炎患者外周血单个核细胞IFN-γ mRNA的表达。结论螺旋藻多糖对慢性乙型肝炎患者外周血单个核细胞的免疫功能具有调节作用。
목적：관찰라선조다당대만성을형간염환자외주혈단개핵세포면역공능적영향。방법운용MTT법화류식세포술분별측정라선조다당대만성을형간염환자외주혈단개핵세포증식능력화세포주기적영향,채용ELISA법검측외주혈단개핵세포배양상청세포인자IFN-γ、IL-2화IL-4농도,동시용RT-PCR법검측외주혈단개핵세포 IFN-γ mRNA적표체。결과라선조다당능증강만성을형간염환자외주혈단개핵세포적증식능력,능증강만성을형간염환자외주혈단개핵세포분비IFN-γ화IL-2,차능강저분비IL-4。라선조다당능상조만성을형간염환자외주혈단개핵세포IFN-γ mRNA적표체。결론라선조다당대만성을형간염환자외주혈단개핵세포적면역공능구유조절작용。
Aim To investigate the immunoregulative action of polysacchrides of Spirulina platensis ( PSP ) on peripheral blood mononuclear cells ( PBMCs) in pa-tients with chronic hepatitis B virus infection. Meth-ods Proliferation and cycle of PBMCs from patients with chronic hepatitis B virus infection were measured by MTT colorimetry and flow cytometry respectively. The concentration of IFN-γ, IL-2 , IL-4 in supernatants of PBMCs were examined by ELISA. The mRNA ex-pression of IFN-γin PBMCs from patients with chronic hepatitis B virus infection was detected by RT-PCR. Results PSP could induce proliferation of PBMCs in patients with chronic hepatitis B virus infection. In ad-dition, PSP could increase the protein expression of IFN-γ and IL-2 in supernatants of PBMCs in patients with chronic hepatitis B virus infection, and PSP could also enhance the mRNA expression of IFN-γof PBMCs in patients with chronic hepatitis B virus infection. However, PSP inhibited the protein expression of IL-4 of PBMCs in patients with chronic hepatitis B virus in-fection. Conclusion PSP can promote the immune function and plays an important immunoregulation function on patients with chronic hepatitis B virus in-fection.